银染端粒重复序列扩增法检测肝癌模拟肝穿组织端粒酶活性的研究

目的：探讨细针穿刺肝活检组织行端粒酶活性检测用于肌细胞癌（HCC）诊断的可行性。方法：HCC共28例，每例HCC肿瘤切除后立即得过且过上肿瘤模拟细针穿刺，应用非放射性同位素银染的端粒重复序列扩增法（TRAP），分别对28例模拟肝穿组织和28例肿瘤切取组织行端粒酶活性检测。结果：模拟肝穿组织与肿瘤切取组织的端粒酶有同等的效果。该结果提示，B超引导下经皮肝穿刺活检行端粒酶活性检测在HCC的诊断，治疗效果评价和预后的判断方面具有一定的应用前景。

Detection of Telomerase Activity in Simulative Fine-Needle Biopsy Tissues of Hepatocellular Carcinoma by Non-Radioisotopic Silver Staining Telomeric Repeat Amplification Protocol

Objective:To explore the feasibility of detection of telomerase activity in fine-needle biopsy tissues of hepatocellular carcinoma (HCC).Methods:Simulative fine-needle biop-sies were performed in28HCC nodules immediately after surgical resection.Telomerase activity of28fine-needle biopsy specimens and28incisional biopsy specimens were detected by non-ra-dioiso topic silver staining telomeric repeat amplification protocol(TRAP).Results:No difference was found between fine-needle biopsy specimens and incisional biopsy specimens with the same telomerase-positive rate(82%)in both groups.Conclusion:Both methods have the same effect for detection of telomerase activity,suggesting that detection of telomerase activity in tumorous tissues obtained by percutaneous fine-needle liver biopsy under the guidance of B-ultrasound should be feasible for diagnosis,assessment of curative effect and judgement of prognosis of HCC.