筋骨草提取物抑制乳腺癌转移与MMPs和TIMPs 表达的关系研究

目的:探讨筋骨草提取物(HBG)对乳腺癌及转移的抑制作用,及其对与乳腺癌侵袭和转移密切相关的基质金属蛋白酶及其抑制物的影响.方法:采用小鼠乳腺癌细胞株4T1原位接种制作乳腺癌自发转移模型,给予50～200 mg· kg-1HBG后评价各组肿瘤生长和肺转移情况;并用免疫印迹法测定肿瘤原位灶和转移灶MMP-2/9,TIMP-1/2的表达水平.结果:50 ～200 mg·kg-1HBG呈剂量依赖性抑制乳腺癌生长和肺转移;下调MMP-2和MMP-9的表达;上调TIMP-1和TIMP-2表达.结论:筋骨草提取物通过调节乳腺癌细胞MMPs和TIMPs的表达而发挥抗乳腺癌及其转移的作用.

Correlation between antimetastatic action of Ajuga decumbens and expression of MMPs and TIMPs

Objective: To investigate the anticancer and anti-metastatic effect of Ajuga decumbens extraction (HBG) on breast cancer and to clarify the effect of HBG on MMPs and TIMPs. Method: The antitumor and antimetastic effect of HBG was determined using orthotopic 4T1 breast cancer mouse model. Western blot analysis was employed to detect the expression of associated proteins in breast cancer metastasis. Result: Administration with 50-200 mg·kg^-1 doses of HBG significantly reduced the tumor weight, tumor volume and numbers of lung tumor nodules in a dose-dependent manner. Tumor metastasis correlated proteins were altered following HBG treatment, MMP-2 and MMP-9 were down-regulated while TIMP-1 and TIMP-2 were up-regulated. Conclusion: HBG showed anticancer and antimetastatic effect towards breast cancer through regulating the expression of MMPs and TIMPs. These data sustain our contention that HBG might be used as a potential therapeutic agent.