注射用雷替曲塞对胃癌患者癌细胞的影响及作用机制研究

目的:了解注射液雷替曲塞对胃癌患者癌细胞的影响及作用机制.方法:将购入的84只裸鼠建立人胃癌细胞MGC-803皮下移植瘤模型,建模成功后随机分为3组,常规组给予腹腔注射生理盐水,雷替曲塞组给予腹腔注射雷替曲塞,5-Fu组给予腹腔注射5-Fu,详细观察对比干预后裸鼠体重、移植瘤体积、瘤重、抑瘤率、凋亡率及癌细胞的周期分布情况.结果:常规组裸鼠体重、移植瘤体积、瘤重均高于雷替曲塞组与5-Fu组,差异有统计学意义(P<0.05);雷替曲塞组裸鼠体重高于5-Fu组,差异无统计学意义(P>0.05),雷替曲塞组移植瘤体积、瘤重低于5-Fu组,差异有统计学意义(P<0.05);常规组裸鼠癌细胞S期分布高于雷替曲塞组与5-Fu组,差异有统计学意义(P<0.05);雷替曲塞组与5-Fu组S期分布,差异无统计学意义(P>0.05);雷替曲塞组与5-Fu组G0/G1,差异无统计学意义(P>0.05);雷替曲塞组与5-Fu组G0/G1期分布低于常规组,差异有统计学意义(P<0.05);三组G2/M期分布无明显差异,差异无统计学意义(P>0.05);常规组癌细胞抑瘤率、凋亡率明显低于雷替曲塞组与5-Fu组,差异有统计学意义(P<0.05);雷替曲塞组癌细胞抑瘤率、凋亡率显著高于5-Fu组,差异有统计学意义(P<0.05).结论:胃癌患者给予雷替曲塞治疗与5-Fu抑瘤效果相似,可有效抑制人胃癌细胞MGC-803裸鼠移植瘤生长,并可诱导癌细胞凋亡.

Study on the Effect and Mechanism of Injection of Raltitrexed on Cancer Cells in Gastric Cancer Patients

Objective:To investigate the effect and mechanism of injection of raltitrexed on cancer cells in gastric cancer patients. Methods:84 nude mice were purchased to establish the human gastric cancer cell MGC-803 subcutaneous tumormodel,after successful modeling,micewere randomly divided into 3 groups,the conventional group received intraperitoneal injection of saline,the raltitrexed group were given intraperitoneal injection of raltitrexed,the 5-Fu group were given intraperitoneal injection of 5-Fu,nude miceweight,tumor volume,tumorweight ,inhibition rate,apoptosis rate, cycle distribution of cancer cellsafter intervention were observed. Results:Nude miceweight,tumor volume,tumor weight of the conventional groupwere significantly higher than those of the raltitrexed group and the 5-Fu group,the difference had statistical significance ( P<0. 05);The raltitrexed group'snude mice weight was higher than that of the 5-Fu group, the difference had no statistical significance ( P>0.05),tumor volume,tumor weight of the raltitrexed group were lower than those of the 5-Fu group,the difference had statistical significance( P<0.05);The nude mice cancer cells S phase distribution of the conventional group was significantly higher than that of the raltitrexed group and the 5-Fu group,the difference had statistical significance(P<0.05); The S phase distribution of the raltitrexed group and the 5-Fu group had no significant difference, the difference hadno statistical significance (P>0.05);There was no significant difference between the raltitrexedgroup and the 5-Fu group in G0/G1,the difference hadno statistical significance ( P>0.05);G0/G1 phase distribution of the raltitrexed group and the 5-Fu group was lower than that of the conventional group, the difference had statistical significance (P<0.05); G2/M phrase distribution among the three groups has no obvious difference, the difference hadno statistical signifi-cance(P>0.05);The conventional group's cancer cell inhibition rate, apoptosis rate were significantly lower than those of the raltitrexed group and the 5-Fu group, the difference had statistical significance( P<0.05);While those of the raltitrexed group were significantly higher than those of the 5-Fu group,the difference had statistical significance ( P<0.05) . Conclusion:Adoptingraltitrexed for gastric cancer patients has similaranti-tumor effect with 5-Fu,which can effectively inhibit the growth of human gastric cancer cell MGC-803 xeno-graft tumor in nude mice and induce apoptosis of cancer cells.