灵芝多糖对多柔比星诱导心肌细胞损伤保护作用的研究

目的:研究灵芝多糖对多柔比星所致心肌损伤的保护作用,探讨多柔比星对心肌细胞损伤的机制.方法:将不同浓度多柔比星(1、2、4、6μmol/L)与H9C2心肌细胞共培养6、12、24h,计算不同作用时间及药物浓度的细胞抑制率;将H9C2心肌细胞与不同质量浓度(0,3.125,6.25,12.5,25mg/L)的灵芝多糖共培养,1h后加入多柔比星,24h后观察细胞形态变化,并计算细胞抑制率;将灵芝多糖与MCF-7,KB,K562共培养24h,1 h后加入多柔比星,48h后计算细胞生长抑制率.结果:2μmoL/L多柔比星与心肌细胞共培养24h的细胞抑制率最高,25mg/L灵芝多糖可明显降低多柔比星诱导的心肌细胞死亡,且不降低药物抗肿瘤作用.结论:灵芝多糖对心肌细胞具有直接保护作用,且不减弱多柔比星抗肿瘤作用.

Study on the Protective Effect of Ganoderma Lucidum Polysaccharides about Doxorubicin Inducing Myocardial Cell Injury

Objective:To study the protective effects of Ganoderma lucidum polysaccharides on adriamy-cin induced myocardial injury,and to explore the mechanism of adriamycin induced myocardial cell.Methods:Different concentrations of doxorubicin ( 1, 2, 4, 6μmol/L) with H9C2 were cultured for 6, 12, 24h,then calculated the cell inhibitory rates at different time and concentration;H9 C2 cardiac myocytes were cultured with Ganoderma lucidum polysaccharides of different mass concentrations (0,3.125,6.25,12.5,25mg/L),adding doxorubicin after 1h,observing morphological changes of the cells after 24h,calculating the cell inhibi-tion rate;MCF-7,KB,K562 were cultured with Ganoderma lucidum polysaccharides,adding doxorubicin after 1h,after 48h calculating the cell inhibition rate Results: The inhibition rate of 24h was the highest when 2μmol/L was cocultured with cells,25mg/L Ganoderma lucidum polysaccharides can significantly reduce the death of myocardial cells induced doxorubicin,and not reduce the anti-tumor effect of drugs. Conclusion:GL-PS has a protective effect on myocardial cells,had no effect on adriamycin inhibit the growth of tumor cells,there is no synergistic effect.