人肝癌组织血管内皮生长因子及微血管密度分析的临床价值

目的 探讨血管内皮生长因子(VEGF)在肝癌(HCC)微血管形成、生长和转移方面的作用。 方法 以免疫组织化学法分析36例HCC组织中VEGF表达状态和细胞内分布,采用微血管染色方法测定微血管密度(MVD),并定量检测癌及癌周组织中总RNA和VEGF水平。 结果 所有HCC组织中VEGF阳性率为63.9％,无包膜组为78.3％,伴有远处转移组为90.9％;VEGF表达与MVD密切相关(t=4.49,,P<0.01);HCC组织VEGF水平或MVD值,在肿瘤直径大小组及肿瘤分化程度高低组间差异无显著性;HCC组织总RNA水平低于癌旁及远癌组织,而VEGF水平明显高于癌旁和远癌组织(q=6.10,P<0.01)。 结论VEGF过度表达和MVD异常是反映HCC侵润生长及转移的有效指标。

Clinical values of vascular endothelial growth factor expression and microvascular density analysis in liver cancer specimens

Objective To explore the roles of vascular endothelial growth factor (VEGF) in microvessel angiogenesis, development and metastasis of hepatocellular carcinoma (HCC). Methods The cellular distributions of VEGF expression and microvascular density (MVD) in 36 HCCs were investigated, and the levels of total RNA and VEGF were detected in HCCs, Para cancerous, and distal cancerous tissues, respectively. Results The incidence of VEGF was 63.9% in 36 cases of HCCs, 78.3% in non-encapsulated HCCs, and 90.9% in HCCs with extrahepatic metastasis, respectively. The VEGF expression was tightly correlated with MVD (t = 4.49, P < 0.01). No significant difference was found between VEGF or MVD and tumor diameter or differentiation degree. The level of total RNA in HCCs was lower but the VEGF level significantly higher than those of Para cancerous or distal cancerous ones (q = 6.10, P < 0.01). Conclusions The present data suggest that VEGF over expression and MVD abnormality are useful markers for vascular invasion and metastasis of liver tumors.