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| 结核分枝杆菌RD1区Rv3873基因真核表达载体的构建及鉴定 | |
| 引用本文： | 曾林子,陈建平,刘成君,李红霞,姚卫,杨志荣.结核分枝杆菌RD1区Rv3873基因真核表达载体的构建及鉴定[J].现代预防医学,2007,34(11):2001-2003. |
| 作者姓名： | 曾林子 陈建平 刘成君 李红霞 姚卫 杨志荣 |
| 作者单位： | 1. 四川大学生命科学院生物资源与生态环境教育部重点实验室,成都,610065 2. 华西医学中心基础医学与法医学院寄生虫教研室 3. 四川绵阳市疾病预防控制中心 |
| 基金项目： | 国家自然科学基金;四川省学术与技术带头人培养基金 |
| 摘 要： | 目的]构建结核分枝杆菌RD1区Rv3873基因的真核表达载体pcDNA3-Rv3873，并对其进行鉴定。方法]利用PCR技术从结核杆菌H37Rv中扩增Rv3873基因，并将其定向克隆至原核载体pGEx-4T-1中，经测序鉴定后，将重组质粒pGEX-4T-1-Rv3873中的目的基因亚克隆入真核表达载体pcDNA3．1（＋），构建重组子pcDNA3．1-Rv3873，通过限制性内切酶切酶及PCR进行鉴定。结果]克隆的Rv3873基因序列与GenBank公布的一致性为100％，限制性内切酶酶切及PCR分析表明Rv3873已成功插入pcDNA3．1（＋）中。结论]成功构建了Rv3873基因真核表达载体，为进—步研究新型结核杆菌DNA疫苗奠定了基础。 |
| 关 键 词： | 结核分枝杆菌 真核表达载体 |
| 文章编号： | 1003-8507（2007）11-2001-03 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-08-01 |
CONSTRUCTION AND IDENTIFICATION OF MYCOB ACTERIUM TUBERCULOSIS Rv3873 FROM RD1 EUKARYOTIC EXPRESSION VECTOR |
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| ZENG Lin-zi, CHEN Jian-ping, LIU Cheng- jun,et al..CONSTRUCTION AND IDENTIFICATION OF MYCOB ACTERIUM TUBERCULOSIS Rv3873 FROM RD1 EUKARYOTIC EXPRESSION VECTOR[J].Modern Preventive Medicine,2007,34(11):2001-2003. | |
| Authors: | ZENG Lin-zi CHEN Jian-ping LIU Cheng- jun |
| Institution: | Key LabOratory of Ministry of Education for Biological Resources and Ecology Environment, Chengdu 610065, China |
| Abstract: | Objective]To construct and identify the eukaryotic expression vector of Mycobacterium tuberculosis Rv3873. Methods]Rv3873 was amplified by PCR and directly cloned into expression plasmid pGEX-4T-1 for sequencing. The target gene in the recombinant plasmid(pGEX-4T-1-Rv3873)was sub-cloned into the eukaryotic expression vector pcDNA3.1( )and the recombinant pcDNA3.1-Rv3873. This recombinant plasmid was identified by restriction analysis and PCR.Results]Comparison of the sequence of Rv3873 gene with that reported in GenBank showed that the identities were 100%.And it was comfirmed that Rv3873 gene was successfully inserted into pcDNA3.1( ).Conclusion]We successfully construct the eukaryotic expression vector pcDNA3.1-Rv3873 and it lays a good foundation for the research of new DNA vaccine of Mycobacterium tubereulosis. |
| Keywords: | RD1 Rv3873 |
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