| 从石蜡包埋和甲醛浸泡组织中提取DNA进行PCR扩增 |
| |
| 引用本文： | 孙文东,邓国仁. 从石蜡包埋和甲醛浸泡组织中提取DNA进行PCR扩增[J]. 农垦医学, 1991, 0(2) |
| |
| 作者姓名： | 孙文东 邓国仁 |
| |
| 作者单位： | 石河子医学院生物化学教研室，北京市肿瘤防治研究所生化室 |
| |
| 摘 要： | 对石蜡色埋组织用二甲苯或水浴法脱石蜡,乙醇进行梯度水化,溶液A裂解细胞,对经抽提、纯化后的DNA进行PCR扩增,获得了较满意的效果。同时对石蜡包埋组织与甲醛浸泡组织中的DNA受损程度进行了比较,此工作为能使肿瘤等症病将在基因水平上进行回顾性分析提供了一个良好开端.
|
| 关 键 词： | 石蜡包埋病理标本 甲(?)浸泡病理标本 DNA提取 PCR扩增 |
THE RESEARCH ON PCR AMPLIFICATION OF DNA EXTRACTED FROM PARAFFIN-EMBEDDED AND FORMALIN-IMMERSED PATHOLOGY SPECIMENS |
| |
| Sun Wendong. THE RESEARCH ON PCR AMPLIFICATION OF DNA EXTRACTED FROM PARAFFIN-EMBEDDED AND FORMALIN-IMMERSED PATHOLOGY SPECIMENS[J]. Agricultural Reclamation Medicine, 1991, 0(2) |
| |
| Authors: | Sun Wendong |
| |
| Abstract: | The pathology specimens of paraffin--embedded and formalin immersed were deparaffinized by adding xylene or by water hath, and rehy- drated ethanol, of which the concentration decreased by degrees. The tinal was resuspended in Solution A and cells were splited The purified DNA was able to be replicated by using PCR method. All above showed us a satisfying resut. In addition, n coparasion was made between DNA of Paraffin-embe- dded specimens and that of furmalin-immersed ones. This report with provide us a good beginning for the reviewable research on tumour diseases at gene level. |
| |
| Keywords: | paraffin emoedded pathology specimens formal(?)n-(?)mmersed pathology specim extraction of DNA PCR amplification |
| 本文献已被 CNKI 等数据库收录！ |
