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| 脐带血清体外培养人骨髓间充质干细胞的生物学特性研究 | |
| 引用本文： | 旷文勇,周新伏,李睿娟,郑敏翠,张广森.脐带血清体外培养人骨髓间充质干细胞的生物学特性研究[J].中国医师杂志,2009,11(10):1317-1320. |
| 作者姓名： | 旷文勇 周新伏 李睿娟 郑敏翠 张广森 |
| 作者单位： | 1. 湖南省儿童医院血液内科,湖南,长沙,410007 2. 湖南省湘潭市中心医院血液内科 3. 中南大学湘雅二医院血液内科 |
| 基金项目： | 湖南省科技厅重点支持项目 |
| 摘 要： | 目的观察脐带血清体外培养人骨髓间充质干细胞的生物学特性。方法抽取骨髓穿刺液10ml，采用含10％脐带血清的DMEM／F12培养，流式细胞仪检测细胞表面抗原，成骨诱导体系及脂肪诱导体系诱导BM—MSCs定向分化。结果BM—MSC—Ss高表达CD29、CD73、CD105，不表达CD34、CIM5、CD31，BM—MSCs体外能诱导为成骨细胞和脂肪细胞。结论脐带血清培养的BM—MSCs的具有较高的纯度和体外分化能力，脐带血清培养BM—MSCs适合临床应用。 |
| 关 键 词： | 胎血 细胞培养技术/方法 造血干细胞 骨髓细胞 |
The biological characteristics of adult human marrow mesenchymal stem cells cultured in umbilical cord serum |
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| Institution: | KUANG Wen-yong, ZHOU Xin-fu, LI Rui-juan, ZHENG Min-cui, ZHNAG Guang-sen( The Children＇s Hospital of Hunan, Changsha 410007, China) |
| Abstract: | Objective To observe biological characteristics of adult human marrow mesenchymal stem cells(BM-MSCs)cultured by the umbilical cord serum.Methods BM-MSCs were obtained from fresh adult human bone marrow and cultured with DMEM/F12 which included 10%umbilical cord blood serum.The surface antigens of BM-MSCs were detected by flow cytometry.BM-MSCs were cultured into differentiation of osteoblasts and adipocytes with conditioned medium for osteogenesis and adipogenesis. Results The antigen of CD29.CD73,and CD105 were high expressed on BM-MSCs.The antigen of CD34,CD45 and CD31 were not expressed on BM-MSCs.The BMMSCs were differentiated into osteoblasts and adipocytes.Conclusion BM-MSCs have high purity and differentiation potency under cuhuring with umbilical cord blood serum.The umbilical cord serum is adapted to clinical application. |
| Keywords: | Fetal blood Cell culture techniques/MT Hematopoietic stem cells Bone marrow cells |
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