| 磷酸钙骨水泥复合微小颗粒骨的体外实验研究 |
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| 引用本文： | 梁建平,刘兴炎,葛宝丰,陈克明,熊发明,李荣刚. 磷酸钙骨水泥复合微小颗粒骨的体外实验研究[J]. 中国矫形外科杂志, 2010, 18(1): 63-66 |
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| 作者姓名： | 梁建平 刘兴炎 葛宝丰 陈克明 熊发明 李荣刚 |
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| 作者单位： | 1. 兰州大学第二临床医学院,兰州,730000
2. 兰州军区兰州总医院骨科,兰州,730050 |
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| 基金项目： | 全军“十一五”医药卫生科研基金资助项目(编号:06MA090); 甘肃省自然科学基金资助项目(编号:0710RJZA068) |
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| 摘 要： | [目的]进行磷酸钙骨水泥复合微小颗粒骨的实验,探讨磷酸钙骨水泥(calcium phosphate cement,CPC)和微小颗粒骨最佳混合比例。[方法]取大耳白兔双侧股骨制作颗粒骨,实验组为颗粒骨和CPC分别以1∶1、1∶2、1∶3、1∶4、1∶5混合固化的材料;对照组为CPC固化的材料。测定两组的固化时间及最大抗压强度;间充质干细胞培养于材料浸提液中,MTT检测细胞增殖;间充质干细胞与各材料共培养5 d后,电镜观察材料表面的细胞量及形态。[结果]MTT检测:比例为1∶2和1∶3组明显促进细胞的增殖,优于其他组,有统计学意义(P0.05)。电镜扫描:1∶2、1∶3组材料表面的细胞生长良好,其他组材料表面的细胞量明显少于这两组。1∶3组材料的固化时间及最大抗压强度适中,与CPC组比较,有统计学意义(P0.05)。[结论]1∶3在可操作性、生物力学、细胞相容性方面是最合适的混合比例。
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| 关 键 词： | 磷酸钙骨水泥 微小颗粒骨 间充质干细胞 组织工程 |
Experimental study of calcium phosphate cement and combined morselized bone in vitro |
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| LIANG Jian-ping,LIU Xing-yan,GE Bao-feng,et al.. Experimental study of calcium phosphate cement and combined morselized bone in vitro[J]. Orthopedic Journal of China, 2010, 18(1): 63-66 |
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| Authors: | LIANG Jian-ping LIU Xing-yan GE Bao-feng et al. |
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| Affiliation: | LIANG Jian-ping,LIU Xing-yan,GE Bao-feng,et al.The Second Clinical Medical College,Lanzhou Univercity,Lanzhou 730000,China |
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| Abstract: | [Objective]To study the combination of calcium phosphate cement(CPC) and morselized bone and their optimum mixing ratio. [Methods]Bilateral nemora of macro-ears rabbits were made into morselized bone.There were two groups:experimental group and control group.In the experimental group,the ratios of morselized bone to CPC were 1 to 1,1 to 2,1 to 3,1 to 4 and 1 to 5.In the control group CPC was used after it was solidified.The maximum compression strength and solidified time of the materials in experimental an... |
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| Keywords: | calcium phosphate cement micromorselized bone mesenchymal stem cells tissue engineering |
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