PELA幽门螺杆菌口服疫苗微球黏膜免疫研究

目的：应用复乳挥发法制备PELA泛影葡胺显影微球与缨门螺杆菌超声上清口服疫苗微球，并进行靶向与黏膜免疫研究。方法：采用CT技术，研究显影微球的靶向，PELA幽门曙杆菌超声上清口服疫苗微球口服免疫小鼠后，运用ELISA法检测血清，唾液，肠粘液的抗体改变情况，ELISPOT法分析派伊尔氏结（PP结）抗原特异性抗体形成细胞（ASC）的数量增减，结果；口粒径在10um以下的微球，首先粘附在胃肠黏膜表面，后投递于PP结；H.pylori疫苗微球免疫后可诱导较高的唾液sIgA水平和肠道sIgA反应，PP结抗原特异性抗体形成细胞（ASC）数量与肠道 sIgA水平密切相关，结论：可生物降解的PELA微球可用于靶向口服疫苗的研究。

Preparation of poly(D,L-lactide) and polyethylene glycol(PELA) microspheres loaded H.pylori lysates and induction of mucosal response in BALB/c mice by oral immunization

Objective To prepare of poly(D,L lactide) and polyethylene glycol(PELA) microspheres loaded H.pylori lysates or Cystografin by double emulsion evaporation and to study the mucosal immune responses.Methods The microspheres loaded Cystografin were used to observe their targeting gastrointestinal mucous membrane by CT, while the microspheres loaded H.pylori lysates were used to immune Balb/c mice by oral administration. The antibody production in salivary and gut washing and antibody secreting cells in Peyer's Patches(PP) was assayed by ELISA and ELISPOT, respectively. Results Oral immunizations induced more significant H.pylori specific intestinal IgA response as well as salivary IgA response than those detected with unencapsulated soluble antigen( P <0.05).The presence of antibody secreting cell in PP was correlated with IgA level in gut washing.These data suggested that PELA Hp could induce H.pylori specific mucosal immune responses in vivo and locally synthesized specific sIgA antibodies. Conclusion The bio degardable PELA microspheres can be used to design targeting oral vaccine in the future. [