Vascular smooth muscle contraction/relaxation of rat carotid artery is not altered by bone grafting substitutes in vitro

Purpose

The aim of this study was to explore the effects of various bone grafting substitutes (Osteosponge®, Perioglas®, Tutoplast ®, and Surgibone®) on vascular smooth muscle tonus.

Methods

Bilateral carotid arteries were removed from rats and contraction/relaxation of isolated vessel rings were measured before and after contact with the biomaterials and then, for dose-dependent epinephrine and papaverin administrations, by a force displacement transducer. The data of each biomaterial group were collected by a computerized system and corresponding software at a sample rate of 1,000 kHz and were converted to contraction force.

Results

Vascular contraction forces were influenced in response to biomaterials tested except for Osteosponge (P?P?>?0.05). There was a dose-dependent vascular response to epinephrine and papaverine administration upon biomaterial contact (P?P?Conclusions Osteosponge®, Perioglas®, Tutoplast ®, and Surgibone® do not alter vascular smooth muscle tonus and vitality and therefore would, presumably, not jeopardize the angiogenesis of fresh blood vessels and full vascularization during tissue healing.