| 广东汉族人PCSK9基因E670G位点多态性与冠心病相关性研究 |
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| 引用本文： | 孟艳辉,刘忠民.广东汉族人PCSK9基因E670G位点多态性与冠心病相关性研究[J].热带医学杂志,2011,11(2):137-140. |
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| 作者姓名： | 孟艳辉 刘忠民 |
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| 作者单位： | 广州医学院第一附属医院医学检验系,广东广州,510120 |
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| 基金项目： | 广东省高等学校重点扶持学科资助项目 |
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| 摘 要： | 目的通过对中国广东地区冠心病(CAD)患者和健康个体前蛋白转化酶枯草杆菌蛋白酶/西布曲明9a型(PCSK9)基因E670G位点单核苷酸多态性(SNP)的研究,分析其在CAD患者和健康人中的分布,以及与冠心病和血脂代谢的相关性。结论采集经冠脉造影确诊的CAD患者血液标本165例,健康人血液标本180例,分别提取基因组DNA模板,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析PCSK9基因E670G位点SNP,并用基因测序法验证结果。血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)浓度水平采用酶法测定。结果中国广东地区CAD患者和健康人群在该位点的等位基因频率、基因型频率分布差异无统计学意义(P>0.05)。CAD患者组和对照组之间TC、LDL-C、HDL-C水平差异具有统计学意义(P<0.05),TG水平差异无统计学意义(P>0.05)。CAD患者组中AA和AG基因型之间LDL-C、HDL-C的差异具有统计学意义(P<0.05),TC、TG的差异没有统计学意义(P>0.05);与非670G携带者比较,670G携带者血LDL-C水平增高,血HDL-C水平降低。AG基因型的冠脉病变支数明显高于AA基因型,其差异具有统计学意义(P<0.05)。结论 PCSK9基因E670G多态性与血LDL-C、HDL-C水平,以及冠心病病变严重程度相关联。
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| 关 键 词： | 冠心病 PCSK9基因 E670G 低密度脂蛋白胆固醇 |
A study on the correlation between E670G polymorphism of PCSK9 gene and coronary artery disease in the Guangdong population |
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| MENG Yan-hui,LIU Zhong-min.A study on the correlation between E670G polymorphism of PCSK9 gene and coronary artery disease in the Guangdong population[J].Journal Of Tropical Medicine,2011,11(2):137-140. |
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| Authors: | MENG Yan-hui LIU Zhong-min |
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| Institution: | MENG Yan-hui,LIU Zhong-min(Department of Medical Laboratory Science,Guangzhou Medical College,Guangdong,Guangzhou 510120,China) |
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| Abstract: | Objective Examined the distribution of a known SNP(23968A>G) of PCSK9 gene in Chinese patients with coronary artery disease(CAD) and healthy people in Guangdong area by PCR-RFLP, and analyzed the association of these SNPs with lipid metabolism in patients with CAD. Methods Blood samples from 165 cases of CAD diagnosed by coronary angiography and 180 cases of normal blood samples were studied.Genomic DNA was amplified by PCR.The amplified product was verified by DNA sequencing.The levels of TC,TG,LDL and HDL... |
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| Keywords: | E670G |
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