维替泊芬介导的光动力疗法治疗兔眼角膜新生血管

目的:旨在探讨和评估光敏剂维替泊芬(vertporfin)介导的光动力疗法(PDT)对角膜新生血管(CoNV)治疗的效果。方法:新西兰白兔随机分为2组:两组采用角膜基质层缝线的方法诱导CoNV形成。Ⅰ组行vertporfin-PDT治疗CoNV,verteporfin以1.5mg/kg静注。Ⅱ组不治疗,为阳性对照。治疗后裂隙灯显微镜观察CoNV变化并记录面积,取处理组及对照组的角膜、虹膜睫状体组织,行组织病理学检查,免疫组织化学(SABC法)检测血管内皮生长因子(VEGF)在角膜组织中的表达。结果:治疗后3d;1,2wk,Ⅰ组CoNV面积均明显小于Ⅱ组(P<0.01)。组织病理学检查显示,Ⅰ组CoNV管壁破坏,形成血栓。VEGF的表达Ⅰ组明显低于Ⅱ组(P<0.01)。结论:Vertporfin-PDT对兔模型眼CoNV有明显的抑制作用,不损伤邻近的正常血管及组织。

Photodynamic therapy with verteporfin in rabbit model for corneal neovascularization

AIM:To investigate and assess the efficacy of photodynamic therapy (PDT) with verteporfin (Visudyne; Novartis AG, Basel, Switzerland) for treatment of corneal neovascularization (CoNV) in rabbit eye model. METHODS:New Zealand rabbits were randomly devided into 2 groups. Corneal neovascularization was induced in rabbits by placing an intrastromal silk suture near the limbus in 2 groups. Group Ⅰreceived PDT in which verteporfin was administered by intravenous injection at a dose of 1.5mg/kg. Group Ⅱ was a positive control group. The change of CoNV was observed with slit lamp microscope and areas of CoNV were then recorded after PDT. Corneal and iris/ciliary body were evaluated with histopathology examination and immumohistochemitry (SABC) method which was used to measure the expression of vascular endothelial growth factor(VEGF). RESULTS:On 3 days, 1 week and 2 weeks after PDT, the mean percentages of CoNV areas of group Ⅰ were smaller than group Ⅱ(P<0.01). Histopathology disclosed that vessel walls of CoNV were destroyed and thrombus formed. The expression of VEGF in group Ⅰ obviously decreased compared to group Ⅱ(P<0.01). CONCLUSION:Vertporfin-PDT has obvious inhibition on CoNV of rabbit model with no damage of normal and tissue around.