| 丁型肝炎病毒在乙肝病毒感染者中分布状况及其分子生物学研究 |
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| 引用本文： | 杨其明 陈健. 丁型肝炎病毒在乙肝病毒感染者中分布状况及其分子生物学研究[J]. 标记免疫分析与临床, 1999, 6(3): 157-160 |
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| 作者姓名： | 杨其明 陈健 |
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| 作者单位： | [1]浙江省宁波传染病医院 [2]宁波市医学科学研究所 |
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| 摘 要： | 通过对丁型肝炎病毒（ＨＤＶ）的放免法检测和巢式ＰＣＲ技术检测，在１２０例乙肝病毒感染者中，检测到１３例ＨＤＶ标记阳性（阳性率为１０．８％），５０例乙肝病毒携带者，３例抗ＨＤＶ－ＩｇＭ阳性。经对照所得乙肝患者同乙肝病毒携带者的ＨＤＶ阳性差异结果支持ＨＤＶ的存在常伴随着谷丙转氨酶（ＡＬＴ）活性上升的观点。实验结果还提示了ＨＤＶ和乙型肝炎病毒（ＨＢＶ）重叠感染易发生重症或慢性肝炎。地ＨＤＶ的检测方法进行
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| 关 键 词： | 乙型肝炎 丁型肝炎 交叉感染 分子生物学 |
Molecular Studies of Hepatitis Deltar Virus RNA Associated with Hepatitis B Virus in Patients |
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| Yang Qiming,Miao Zhenqiu,Ye Junmao et al.. Molecular Studies of Hepatitis Deltar Virus RNA Associated with Hepatitis B Virus in Patients[J]. Labeled Immunoassays and Clinical Medicine, 1999, 6(3): 157-160 |
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| Authors: | Yang Qiming Miao Zhenqiu Ye Junmao et al. |
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| Abstract: | Hepatitis delta virus was detected by RIA and nested-PCR method in 120 patients who were infected with hepatitis B virus. The detection rate of HDV-RNA was 10.8%.The experiments showed that HDV superinfection associated with high ALT levels and HBV infection. And the studies suggested that cross infection of HDV and HBV should be in horizontal and longitudinal patients with high level. We also compared the detection method RIA with nested-PCR and cloned the PCR products.NDA sequence analysis data showed that the sequence of nested-PCR products were HDV-RNA data. |
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| Keywords: | HBV infector HDV PCR RIA |
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