人参二醇皂苷对LPS休克大鼠肺组织AQP1表达的影响

目的：观察内毒素(LPS)诱导的休克大鼠肺泡毛细血管内皮细胞膜水通道蛋白1(AQP1)的表达变化，及人参二醇皂苷（PDS）和糖皮质激素对其的影响。方法：应用HE染色观察肺组织病理学变化，通过免疫组织化学和免疫印迹分析的方法检测AQP1在肺组织中的表达。结果：（1）病理组织学结果显示：模型组（LPS）：肺组织可见较弥漫的间质炎性改变，细支气管周围及血管周围有明显的炎性渗出物，并可见出血，部分肺泡腔内含有水肿液，炎症病变较轻处可见代偿性肺气肿改变。然而，地塞米松组（DEX）、人参治疗组（PDS）大鼠肺脏改变明显减轻。（2）免疫组织化学显示：正常对照组（CTR）肺泡周围毛细血管内皮细胞 AQP1呈阳性表达。LPS组肺泡周围毛细血管内皮细胞AQP1呈微弱表达。DEX组和PDS 3个剂量组大鼠肺泡周围毛细血管内皮细胞 AQP1呈阳性表达，但较正常对照组弱。（3）免疫印迹分析结果显示:LPS组AQP1表达水平明显低于CTR组，PDS 3组及DEX组的AQP1表达水平虽低于CTR组，但明显高于LPS组结论：人参二醇皂苷与地塞米松有减轻LPS诱导的肺损伤作用;LPS具有抑制LPS休克肺AQP1表达的作用，而人参二醇皂苷与地塞米松能使其表达提高，这进一步证实AQP1与肺水肿形成有关。

Effect of panaxadiols on AQP1 expression in lungs of LPS shock rats

AIM: To investigate the alteration of aquaporin 1(AQP1) expression in lung tissues in endotoxic shock rats and the effect of panaxadiols.METHODS: The histological changes were examined by HE staining.The expression of AQP1 was analyzed by immunohistochemical staining and Western blotting.RESULTS:(1) Significant inflammatory changes in pulmonary interstitial in rats of LPS group were observed.However,in dexamethasone(DEX) treatment group and panaxadiols(PDS) treatment group, the pulmonary pathologic changes were much slighter.(2) Positive AQP expression in the endothelium of capillary vessels on the wall of alveolus were seen in control(CTR) group,while merely very weak expression in LPS group was detected. The positive staining in PDS groups and DEX group were significantly stronger than that in LPS group,and close to CTR group.(3) The results of Western blotting showed that the quantity of AQP1 expression in LPS group was significantly lower than that in other groups,also the expression in PDS groups and DEX group was slightly lower than that in control group.CONCLUSION: LPS inhibits the expression of AQP1,while PDS increases the strength of AQP1 expression in lungs of endotoxic shock rats.