ELL慢病毒表达载体的构建及其感染前列腺癌PC3细胞的研究

目的构建ELL基因的慢病毒表达质粒，探讨其感染人前列腺癌PC3细胞的可行性。方法将含有全长ELL的质粒和慢病毒表达载体经双酶切后连接，构建成重组慢病毒载体质粒pCDH1-MCS1-EF1-copGFP—ELL。对慢病毒载体质粒进行双酶切和测序鉴定后，制备包装病毒并转染人前列腺癌细胞系PC3。结果慢病毒载体质粒pCDH1-MCS--EF1-copGFP—ELL的酶切和测序结果与预计的序列一致。慢病毒感染人前列腺癌PC3细胞后能稳定高表达ELL。结论成功构建了ELL的慢病毒表达载体，ELL可被成功转染人人前列腺癌细胞。

Construction of ELL gene lentiviral expression vector and its infection in human prostate cancer PC3 cells

Objective To construct ELL gene lentiviral feasibility of its infection in human prostate cancer PC3 cells expression plasmid and explore the Methods Containing the full-length ELL plasmid and lentivirus vector were double restriction digested and connected to construct recom- binant lentiviral expression vector pCDH1-MCS1-EFI-copGFP- ELI.. Identified by double restriction enzyme digestion and sequencing analysis, recombinant plasmid was packaged and used to infect hu- man prostate cancer PC3 cells. Results Double restriction enzyme digestion and sequencing analy- sis of lentiviral vector plasmid pCDH1-MCSI-EFI-copGFP- ELL showed the expected results. Hu- man prostate cancer PC3 cells infected by lentiviral vector can overexpress ELL protein. Conclu- sions ELL lentiviral expression vector was successfully constructed. ELL can be successfully trans- fected into human prostate cancer cells.