绝经后盆底组织TGF-β_1和CollagenⅠ、Ⅲ表达与SUI的关系

目的:探讨绝经后压力性尿失禁(SUI)患者盆底支持结构转化生长因子β1(TGF-β1)、胶原蛋白Ⅰ、Ⅲ(CollagenⅠ、Ⅲ)与SUI的关系。方法:采用免疫组化方法,测定30例压力性尿失禁患者(SUI组)、28例盆底器官膨出(POP)患者(POP组)阴道前壁组织中TGF-β1及Collagen阳性率,并选择同期30例非卵巢功能性肿瘤和宫颈病变患者作为对照(对照组)。结果:SUI组、POP组及对照组患者阴道前壁组织中,TGF-β1表达阳性率分别为20.00%、14.30%、93.33%,SUI组与对照组比较,差异有极显著性(P<0.01);SUI组与POP组比较,差异无显著性(P>0.05)。3组患者阴道前壁组织中胶原Ⅰ含量的表达以辉度值为标准,在SUI组、POP组及对照组分别为98.62、98.15和100.03。SUI组与对照组比较,差异有极显著性(P<0.01);POP组与对照组比较,差异有极显著性(P<0.01);SUI组与POP组比较,差异无显著性(P>0.05)。3组患者阴道前壁组织中胶原Ⅲ含量的表达以辉度值为标准,在SUI组、POP组及对照组分别为98.46、98.23和100.12。SUI组与对照组比较,差异有极显著性(P<0.01);POP组与对照组比较,差异有极显著性(P<0.01);SUI组与POP组比较,差异无显著性(P>0.05)。结论:绝经后SUI患者盆底支持结构的退行性病变与组织中的转化生长因子β1减少及胶原蛋白含量水平低下有关。

Study on transforming growth factorβ1 and collagen Ⅰ, Ⅲ in paries anterior vaginase for female stress urinary incontinence

Objective:To explore the relationship between transforming growth factorβ1(TGF-β1) and collagen Ⅰ,Ⅲ with the occurrence of stress urinary incontinence(SUI) through analysis on TGF-β1 and collagenⅠ,Ⅲ in paries anterior vaginase.Methods:The biopsy specimens in paries anterior vaginase were obtained from 88 subjects undergoing hysterectomia,tension-free vagina tape(TVT),Burch procedures,who were assigned in the control,pelvic organ prolapse(POP) and SUI groups.The histological structures in paries anterior vaginase were examined with routine HE staining.TGF-β1 and collagen Ⅰ,Ⅲ in structures in paries anterior vaginase was evaluated by immunohistochemical staining.Results:TGF-β1 positive staining rate in paries anterior vaginase was 20.00% in SUI group,14.30% in POP group,93.33% in contral group, respectively.It was significantly lower in SUI group and POP group than that in control group(P<0.05),and it wasn't significantly lower in SUI group than that in POP group(P>0.05) rate.The collagen Ⅰ positive staining luminance in paries anterior vaginase was 98.62 in SUI group,98.15 in POP group,100.03 in contral group,respectively.It was significantly lower in SUI group and POP group than that in control group(P<0.05),and it wasn't significantly lower in SUI group than that in POP group(P>0.05) rate.The collagen Ⅲ positive staining luminance in paries anterior vaginase was 98.46 in SUI group,98.23 in POP group,100.12 in contral group,respectively.It was significantly lower in SUI group and POP group than that in control group(P<0.05),and it wasn't significantly lower in SUI group than that in POP group(P>0.05).Conclusion:There is close relationship between degeneration of pelvic support construction and deficiency in TGF-β1 and collagen Ⅰ,Ⅲ declines in paries anterior vaginase of SUI patient.