膜联蛋白A1在非小细胞肺癌组织中的表达及意义

  目的  检测膜联蛋白A1（annexin A1，ANXA1）在人非小细胞肺癌组织中的表达情况，并探讨其临床意义。  方法  通过实时荧光定量PCR、蛋白免疫印迹和免疫组织化学技术检测非小细胞肺癌患者肺癌组织和癌旁组织（距肿瘤边缘>5 cm）中膜联蛋白A1的表达水平，并分析其与临床病理参数之间的关系。  结果  实时荧光定量PCR显示，膜联蛋白A1 mRNA在肺癌组织中的表达水平（0.574±1.403）高于癌旁组织（0.240±0.893），差异具有统计学意义（t=2.060，P=0.045）。肺癌组织中膜联蛋白A1的表达与其分化程度、淋巴结转移、TNM分期相关（P < 0.05）；与性别、年龄、吸烟史、肿瘤直径、组织学类型无关（P > 0.05）。蛋白免疫印迹和免疫组织化学结果均显示，膜联蛋白A1蛋白在肺癌组织中的表达高于癌旁组织。  结论  ANXA1在非小细胞肺癌患者肺癌组织中呈高表达，可能与其发生发展和侵袭转移有一定关系。 

Expression of annexin A1 in non-small cell lung cancer tissues and its significance

  Objective   This study aimed to detect the expression of annexin A1 in human non-small cell lung cancer (NSCLC) and to explore its clinical significance.   Methods   We detected the expression levels of annexin A1 in NSCLC and the same patient's distal cancerous tissue (from the edge of the tumor >5 cm) by real-time fluorescence quantitative polymerase chain reaction (real-time PCR), Western blot, and immunohistochemical methods, and then analyzed their correlation with clinical pathological parameters.   Results   Real-time PCR showed that the expression of annexin A1 mRNA in NSCLC was higher than that of distal cancerous tissue (0.574±1.403 vs. 0.240±0.893, t=2.060, P=0.045). Moreover, the expression of annexin A1 in NSCLC was associated with the degree of differentiation, lymph node metastasis, and TNM staging (P < 0.05), but independent of gender, age, smoking history, tumor size, and histological type (P > 0.05). Western blot and immunohistochemistry revealed that the expression of annexin A1 protein in NSCLC was higher than that of distal cancerous tissue.   Conclusion   Annexin A1 is highly expressed in the cancer tissues of patients with NSCLC, which may be correlated with the occurrence and development of tumor and its invasion and metastasis.