| DEAE-SephadexA50柱层析分离HbA_2、HbA和HbF的实验研究 |
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| 引用本文： | 吕灿群,崔耀宗,范汝兰,董志明.DEAE-SephadexA50柱层析分离HbA_2、HbA和HbF的实验研究[J].皖南医学院学报,1986(4). |
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| 作者姓名： | 吕灿群 崔耀宗 范汝兰 董志明 |
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| 作者单位： | 皖南医学院生物化学教研室，皖南医学院生物化学教研室，皖南医学院生物化学教研室，皖南医学院生物化学教研室 |
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| 摘 要： | 本文应用 DEAE-Sephadex A50柱层析法从95%正常人红细胞溶血液和5%脐带血红细胞混合液中分离出 HbA_2、HbA 和 HbF。其中 HbA_2 部分经醋纤薄膜电泳检查,发现除含 HbA_2 外,尚含有少量碳酸酐酶。为了进一步纯化,采用SephadexG-75凝胶过滤层析,使 HbA_2 和碳酸酐酶分离。经纯化的 HbA_2 通过聚丙烯酰胺凝胶电泳鉴定,呈现一条 HbA_2 区带,证明 HbA_2 达到聚丙烯酰胺凝胶电泳纯的程度。采用分离纯化的 HbA_2 免疫动物,制取抗 HbA_2 血清,为 HbA_2 免疫检测的研究提供材料。
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| 关 键 词： | 血红蛋白A 血红蛋白A_2 层析分离 碳酸酐酶 聚丙烯酰胺凝胶电泳 |
STUDIES OF HbA_2,HbA AND HbF SEPARATED BY COLUMN CHROMATOGRAPHY |
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| Lu canqun Cui Yaozong Fan Rulan Dong Zhiming.STUDIES OF HbA_2,HbA AND HbF SEPARATED BY COLUMN CHROMATOGRAPHY[J].Acta Academiae Medicinae Wannan,1986(4). |
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| Authors: | Lu canqun Cui Yaozong Fan Rulan Dong Zhiming |
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| Institution: | Department of Biochemistry.Wannan Medical College Wuhu |
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| Abstract: | HbA_2 HbA and HbF were separated by DEAE-sephadeXA50 Column chromat- graphy from the mixture of 95% normal red blood cell hemolysate and 5% cord red blood cell hemolysate,The Separated HbA_2 was found containing carbonic anhydrase which was identified by cellulose acetate strips electrophoresis. The HbA_2was farther Varified with sephadexG-75 appearing to be a band in Polyacylamide gel electrophoresis.Wa have prepared specific HbA_2 antiserum which is believed to be useful for immunoassay HbA_2. |
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| Keywords: | Hemoglobin A/~*IP Hemoglobin A_2/~*IP Chromatography Carbonic anhydrase/~*IP Electroohoresis Polyacrylamide gel |
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