| 人CD146＋牙周膜干细胞的分选及其干细胞特性的鉴定 |
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| 引用本文： | 朱文俊,谭媛元,梁敏.人CD146＋牙周膜干细胞的分选及其干细胞特性的鉴定[J].中华老年口腔医学杂志,2013(2):74-78. |
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| 作者姓名： | 朱文俊 谭媛元 梁敏 |
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| 作者单位： | 中山大学附属口腔医院牙周科,广东510055 |
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| 基金项目： | 国家自然科学基金（编号81170970）;留学归国人员科研启动基金（编号2009134）;广东省科技计划（编号2011B031800259） |
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| 摘 要： | 目的：通过酶消化法及流式细胞分选技术分选CD146＋牙周膜干细胞（Periodontal ligament cells，PDLCs），探讨其干细胞特性。方法：采用流式细胞技术从人牙周膜细胞中分选CD146＋PDLCs，并对其进行免疫组化染色（角蛋白、波形丝蛋白及Stro-1），成骨、成脂诱导分化及克隆形成实验鉴定其干细胞特性。结果：CD146＋PDLCs表达波形丝蛋白及Stro-1，具有成骨及成脂分化能力，体外培养能形成细胞克隆。结论：以CD146为分选指标的流式细胞分选技术可作为牙周膜干细胞筛选的方法。
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| 关 键 词： | 牙周膜细胞 CD146 成骨分化 成脂分化 |
Isolation and identification of human CD146 positive periodontal ligament stem cells |
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| ZHU Wen-Jun,TAN Yuan-yuan,LIANG Min.Isolation and identification of human CD146 positive periodontal ligament stem cells[J].Chinese JOurnal of Geriatric Dentistry,2013(2):74-78. |
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| Authors: | ZHU Wen-Jun TAN Yuan-yuan LIANG Min |
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| Institution: | (Department of Periodontology, Oral Subsidiary Sun Yat-sen University GuangHua Hospital, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China) |
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| Abstract: | Objectives： To isolate and culture CD146 positive periodontal ligament cells （CD146＋PDLCs） by using type I collagenase digestion and flow cytometric sorting, in order to verify the stem cell properties of CD146＋PDLCs. Methods： CD146＋PDLCs were isolated by flow activated cell sorter fi＇om human PDLCs. The stem cell properties of CD146＋PDLCs were tested by immunohistochemical staining, osteogenic/adipogenic induction and colony-forming assay. Results： CD146＋PDLCs, expressing mesenchymal stem cell surface marker Stro-1, were able to form calcium nodules/lipid droplets under osteogenic/adipogenic induction and were capable of forming colonies. Conclusions： CD 146＋PDLCs shared the similar properties with periodontal ligament stem cells, which providing a new method for periodontal ligament stem cell isolation. |
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| Keywords: | periodontal ligament cells CD146 osteogenesis adipogenesis |
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