| 星点设计优选苦参纤维素酶解提取工艺研究 |
| |
| 引用本文： | 杨银花,谢云,吴清,王玉蓉. 星点设计优选苦参纤维素酶解提取工艺研究[J]. 北京中医药大学学报, 2011, 34(8): 552-555,576 |
| |
| 作者姓名： | 杨银花 谢云 吴清 王玉蓉 |
| |
| 作者单位： | 北京中医药大学中药学院 北京100102;北京中医药大学中药学院 北京100102;北京中医药大学中药学院 北京100102;北京中医药大学中药学院 北京100102 |
| |
| 基金项目： | 国家自然科学基金资助项目(No.81073059) |
| |
| 摘 要： | 目的 利用纤维素酶对苦参进行水解,以提高苦参有效成分的提取转移率、节省提取时间.方法 在单因素试验基础上,以HPLC测得的苦参碱、氧化苦参碱的提取转移率为指标,采用星点试验设计,对加酶量（X1）、酶解pH（X2）和酶解时间（X3）3个因素（每个因素设5个水平）进行考察,优选苦参最佳酶解条件,并且以水提取方法作为对比,对...
|
| 关 键 词： | 苦参 纤维素酶 星点设计 提取工艺 |
Enzymatic extractive technique with cellulase optimized with central composite design for Kushen(Radix Sophorae Flavescentis) |
| |
| YANG Yin-hua,XIE Yun,WU Qing,WANG Yu-rong. Enzymatic extractive technique with cellulase optimized with central composite design for Kushen(Radix Sophorae Flavescentis)[J]. Journal of Beijing University of Traditional Chinese Medicine, 2011, 34(8): 552-555,576 |
| |
| Authors: | YANG Yin-hua XIE Yun WU Qing WANG Yu-rong |
| |
| Affiliation: | YANG Yin-hua,XIE Yun,WU Qing,WANG Yu-rong(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102) |
| |
| Abstract: | Objective To carry out the hydrolysis for Kushen(Radix Sophorae Flavescentis) by using cellulose to increase the extraction transfer rate and save extraction time of active principles of Kushen.Methods On the foundation of single factor test,three factors,amount of cellulose addition(X1),pH value of enzymolysis(X2) and time of enzymolysis(X3),were observed(5 levels being set to each factor) by using central composite design taken the extraction transfer rate of matrine and oxymatrine obtained by HPLC as ind... |
| |
| Keywords: | Kushen(Radix Sophorae Flavescentis) cellulase central composite design extractive technique |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
