| 环孢素A对阿霉素治疗肝癌影响的实验研究 |
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| 引用本文： | 张俊峰,陈规划,陆敏强,李华,蔡常洁,杨扬,陈伟. 环孢素A对阿霉素治疗肝癌影响的实验研究[J]. 肿瘤防治研究, 2006, 33(9): 629-631. DOI: 10.3971/j.issn.1000-8578.1328 |
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| 作者姓名： | 张俊峰 陈规划 陆敏强 李华 蔡常洁 杨扬 陈伟 |
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| 作者单位： | 中山大学附属第三医院肝移植中心; 中山大学附属第三医院器官移植实验中心; |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的探讨环孢素A(Cyclosporine A,CsA)对阿霉素(Adriamycin,ADM)治疗肝癌的影响。方法培养HepG-2肝癌细胞,分成对照、CsA、ADM、CsA+ADM组,分别处理24~48h,采用MTT法检测细胞增殖、流式细胞术测定细胞周期及细胞凋亡。结果肝癌细胞增殖能力48h明显受到抑制,CsA在5~1000μg/L随浓度增加,凋亡率逐渐增加,超过该浓度时,则出现凋亡率减低。CsA、及ADM均使G2期显著延长;CsA对HepG-2肝癌细胞有促进细胞凋亡作用,与ADM有协同促调亡作用。结论环孢素A对肝癌增殖有抑制作用,使G2期阻滞,促凋亡。环孢素A联合阿霉素对肝癌治疗有协同作用。
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| 关 键 词： | 肝细胞癌 环孢菌素 阿霉素 细胞增殖 |
| 文章编号： | 1000-8578（2006）09-0629-03 |
| 收稿时间： | 2005-09-07 |
| 修稿时间： | 2005-09-072005-11-12 |
Influence of Combined Cyclosporine A with ADM on Hepatocellular Carcinoma |
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| ZHANG Jun-feng,CHEN Gui-hua,LU Min-qang,LI Hua,CAI Chang-jie,YANG Yang,CHEN Wei. Influence of Combined Cyclosporine A with ADM on Hepatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2006, 33(9): 629-631. DOI: 10.3971/j.issn.1000-8578.1328 |
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| Authors: | ZHANG Jun-feng CHEN Gui-hua LU Min-qang LI Hua CAI Chang-jie YANG Yang CHEN Wei |
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| Affiliation: | 1.The Center of Liver Transplantation of The Third Affiliated Hospital of Sun Yet Sen University; Guangzhou 510630; China; 2.Depatment of The Liver Transplantation Laboratory; |
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| Abstract: | Objective To investigate the influence of combined cyclosporine A with adriamycin on hepatocellular carcinoma. Methods HepG-2 cells were cultured,they were divided into 4 grops namely, control、CsA、ADM、CsA+ADM group, acted on respectively for 24 to 48 hours. Cell growth was assessed with MTT, cell cycle and cell apoptosis were assessed by flow cytometry. Results The ability of growth was inhibited in 48 hour. CsA and ADM could increased cell ratio of G_ 2 phase; the apoptosis ratio was increased when CsA was... |
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| Keywords: | Human hepatocellular carcinoma cell line Cyclosporine A Adriamycin Cell proliferation |
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