子痫前期病人血清可溶性血管内皮生成因子受体1水平的检测

目的探讨子痫前期病人血清可溶性血管内皮生成因子受体1(sFlt-1)水平的变化及其意义。方法采用酶联免疫吸附试验(ELISA)法测定30例子痫前期病人(子痫前期组)产前和产后48 h血清血管内皮生成因子(VEGF)及sFlt-1水平。并以45例健康孕妇作为对照(对照组),其中早期妊娠18例、中期妊娠12例、晚期妊娠15例。晚期妊娠组及子痫前期组均随访至产后(正常产后组、子痫前期产后组)。结果中期妊娠组VEGF水平与早期及晚期妊娠组比较,差异均有统计学意义(t=3.456、2.274,P<0.01、0.05)。早、中期妊娠组sFlt-1水平与晚期妊娠组比较,差异有统计学意义(t=3.494、2.244,P<0.05)。正常产后组VEGF与sFlt-1水平均较晚期妊娠组减低,差异有显著性(t=3.595、2.338,P<0.01、0.05)。子痫前期组血清VEGFs、Flt-1水平与晚期妊娠组比较,差异均有显著性(t=11.785、8.643,P<0.01)。子痫前期产后组VEGFs、Flt-1水平与正常产后组比较差异无统计学意义(t=1.986、1.018,P>0.05)。妊娠32周前发病及伴胎儿生长受限的子痫前期病人血清sFlt-1水平较妊娠32周后发病及不伴胎儿生长受限的子痫前期病人血清sFlt-1水平明显升高(t=2.305、2.286,P<0.05)。子痫前期组血清sFlt-1水平与24 h尿蛋白总量呈明显正相关(r=0.517,P<0.01)。母体血清sFlt-1与VEGF水平呈明显负相关(r=-0.383,P<0.05)。结论子痫前期病人血清sFlt-1水平升高且与子痫前期发病时间和疾病严重程度相关。

DETECTION OF SERUM VEGF AND SOLUBLE FMS-LIKE TYROSINE KINASE 1 IN PREECLAMPSIA

ObjectiveTo observe the level of serum soluble fms-like tyrosine kinase 1(sFlt-1) in patients with preeclampsia and its significance.MethodsThe serum levels of VEGF and sFlt-1 were detected by enzyme linked immuosorbent assay(ELISA) in 30 women with preeclampsia,48 h before and after labour,and 45 normal pregnant women including early pregnancy(18),midtrimester pregnancy(12) and late pregnancy(15).ResultsThe difference of VEGF levels between midtrimester-pregnancy group and early-as well as late-pregnancy group significant(t=3.456,2.274;P<0.01,0.05);the difference of sFlt-1 levels between early-,midtrimester-,and late-pregnancy group was significant(t=3.494,2.244;P<0.05).The levels of VEGF and sFlt-1 of normal postpartum group were lower than those of the late-pregnancy group(t=3.595,2.338;P<0.01,(0.05)).The level of VEGF of the preeclampsia group was significantly lower than that of the late-pregnancy group(t=11.785,(P<)0.01).The level of sFlt-1 of the preeclampsia group was significantly higher than that of the late-pregnancy group(t=8.643,P<0.01).The levels of VEGF and sFlt-1 of the preeclamptic postpartum group were not significantly different compared with the normal postpartum group(t=1.986,1.018;P>0.05);the levels of sFlt-1 of the preeclamptic women with the onset before 32 gestation weeks and with fetal growth retardation were significantly higher than those of the women with the onset after 32 gestation weeks and not with fatal growth retardation(t=2.305,2.286;P<0.05).Positive relationship was observed between the level of sFlt-1 and the 24-hour proteinuria in women with preeclampsia(r=0.517,P<0.01).Negative relationship was seen between the level of sFlt-1 and VEGF in the maternal serum(r=-0.383,P<0.05).ConclusionThe level of sFlt-1 in patients with preeclampsia increases and is associated with the onset time and the severity of the illness.