| Celecoxib通过环氧合酶2和前列腺素E_2途径抑制胰腺癌JF-305细胞增殖 |
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| 引用本文： | 吴高松,邹声泉,. Celecoxib通过环氧合酶2和前列腺素E_2途径抑制胰腺癌JF-305细胞增殖[J]. 肿瘤防治研究, 2003, 30(6): 480-482 |
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| 作者姓名： | 吴高松 邹声泉 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院普外科，武汉430030 |
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| 摘 要： | 目的 研究Celecoxib对COX 2高表达人胰腺癌细胞JF 30 5生长和凋亡的影响及作用机制。方法 采用四唑氮蓝 (MTT)比色法检测细胞增殖 ,流式细胞仪测定细胞周期和凋亡 ,酶联免疫吸附试验 (ELISA)检测前列腺素E2 (PGE2 )含量。结果 Celecoxib可明显抑制JF 30 5细胞增殖和诱导其凋亡 ,并且这种增殖抑制作用能被PGE2 拮抗。结论 Celecoxib通过COX 2和PGE2 途径抑制JF 30 5细胞生长和诱导其凋亡 ;Celecoxib可能是COX 2高表达胰腺肿瘤的一种有效的化学治疗和化学预防药物。
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| 关 键 词： | Celecoxib 环氧合酶-2 前列腺素E2 胰腺癌 JF-305 细胞增殖 Pancreatic carcinoma Cell line Cyclooxygenase-2 (COX-2) Prostaglandin E2 Celecoxib |
| 文章编号： | 1000-8578(2003)06-0480-03 |
| 收稿时间： | 2002-12-10 |
| 修稿时间： | 2002-12-10 |
Celecoxib inhibits proliferation and induces apoptosis via cyclooxygenase-2 andPGE2 pathway in human pancreatic carcinoma cell line JF-305 |
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| WU Gao-song,ZOU Sheng-quan. Celecoxib inhibits proliferation and induces apoptosis via cyclooxygenase-2 andPGE2 pathway in human pancreatic carcinoma cell line JF-305[J]. Cancer Research on Prevention and Treatment, 2003, 30(6): 480-482 |
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| Authors: | WU Gao-song ZOU Sheng-quan |
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| Affiliation: | Department of General Surgery; Tongji Hospital; Tongji Medical College; Huazhong University of Science and Technology; Wuhan 430030; China; |
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| Abstract: | Objective To evaluate the effects and mechanisms of celecoxib in inducing proliferation inhibition and apoptosis on human pancreatic carcinoma cells. Methods The anti-proliferative effect was measured by using Methabenzthiazuron (MTT) assay. Cell cycle and apoptosis were analyzed by using flow cytometry. The prostaglandin E 2 (PGE 2) levels in the supernatant of cultured pancreatic carcinoma cells were quantitated by enzyme-linked immunoabsordent assay (ELISA).Results Celecoxib suppressed the productio... |
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| Keywords: | Pancreatic carcinoma Cell line Cyclooxygenase-2 （COX-2） Prostaglandin E2 Celecoxib |
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