大蒜新素对巨细胞病毒感染小鼠脾细胞TGF-β1和IL-10的抑制作用

目的:研究大蒜新素对巨细胞病毒(CMV)感染鼠的免疫抑制性细胞因子TGF-β1和IL-10的影响,探讨大蒜新素的抗CMV机制。方法:建立非免疫抑制性鼠巨细胞病毒(MCMV)全身播散性感染小鼠模型;双抗体夹心ELISA法检测感染后1,3,7,14,28,45,60,75,90,120d正常对照组、病毒感染组和药物治疗组小鼠脾细胞培养上清中TGF-β1和IL-10水平的时序性变化。结果:MCMV感染后,TGF-β1和IL-10分泌均显著升高,并呈现出急慢性期两次高峰的特点。大蒜新素治疗对MCMV感染鼠各时间点TGF-β1水平均有下调作用,且对其急性期分泌高峰的抑制更明显。大蒜新素治疗对IL-10的急性期分泌高峰影响不明显,却显著下调慢性期IL-10的分泌高峰。结论:大蒜新素能显著抑制MCMV感染后TGF-β1和IL-10分泌,进而增强机体细胞免疫功能而有利于机体清除MCMV病毒,这可能是大蒜新素抗CMV的另一作用机制。

Inhibitory Effect of Allitridin on the Expression of Transforming Growth Factor-Beta 1 and Interleukin-10 in Splenocytes of Cytomegalovirus Infected Mice

Objective:To study the influence of allitridin on the level of immunosuppressive cytokines TGF-β1 and IL-10 in mouse cytomegalovirus(MCMV)infected mice,and to find the mechanisms of allitridin against CMV.Methods:A disseminating infected murine model was established without using immunosuppressant.TGF-β1 and IL-10 level in spleen cell culture supernatants were measured in normal control group,MCMV infected group and allitridin treated group by doubleantibody sandwich ELISA at 1,3,7,14,28,45,60,75,90,and 120 day(s)post infection.Results:The levels of TGF-β1and IL-10 elevated remarkably after MCMV infection,and both showed the feature of two crests in the time-concentration curve.Allitridin could reduce the increasing secretion of TGF-β1in all time points,especially on the first crest.And allitridin had no evident effects on the first crest of IL-10 level,but it could suppress IL-10 secretion significantly on the second crest.Conclusion:Allitridin could inhibit the secretion of TGF-β1 and IL-10 in MC-MV infected mice,which could facilitate the immunity of the host and was helpful for the clearance of MCMV.This might be another protective mechanism of allitridin against CMV.