当归补血汤对大鼠糖尿病足iNOS mRNA表达的影响

目的:探讨当归补血汤对糖尿病大鼠足部病变的保护作用及其机制。方法:SD雄性大鼠腹腔注射链脲佐菌素(STZ)并结合室温控制以建立糖尿病足模型,给予当归补血汤或西洛他唑治疗。实验第3周末时,观察各组肢端坏疽并测定血糖情况。腹主动脉取血,测定血甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、C反应蛋白(CRP)、C肽(CP)、糖化血红蛋白(GHb)。应用反转录多聚酶链反应技术(RT-PCR),观察各组大鼠小腿腓肠肌iNOSmRNA的表达情况。结果:STZ诱导的糖尿病大鼠存在明显的血糖、血脂及CRP、CP、GHb异常(P<0.01或<0.05),且出现不同程度的肢端坏疽。当归补血汤可以降低上述指标,减轻肢端坏疽(P<0.01或<0.05),而西洛他唑只改善了CRP和CP(P<0.01)。经给药处理3周后,当归补血汤组及西洛他唑组大鼠足部iNOSmRNA均有不同程度的下调(P<0.01)。结论:当归补血汤可改善血糖、血脂代谢紊乱,降低异常升高的GHb、CP、CRP水平,减轻肢端坏疽的症状,并能纠正升高的iNOSmRNA的表达,推测其能延缓高糖状态下的足部病变,对糖尿病足有一定的保护作用。

Protective Effect of Danggui Buxue Decoction in Rats with Diabetic Foot

Objective:To explore the protective mechanism of Danggui Buxue Decoction(DBD),a traditional Chinese drug which consists of radix astragali and angelica sinensis,in rats with diabetic foot.Methods:The rat model with diabetic foot was made by intraperitoneal injection of 55 mg/kg streptozotocin(STZ)and temperature control.Rats were treated with DBD or cilostazol.After 3weeks of treatment,blood glucose,lipid,glycohemoglobin(GHb),C-peptide(CP)and C-reactive protein(CRP)were measured,and gangrene of feet was also observed.RT-PCR technique was used to observe the expression of iNOS mRNA.Results:The diabetic rats induced by STZ had abnormal blood levels of glucose,lipid,GHb,CP,and CRP(P<0.01or 0.05),and different degrees of gangrene.DBD alleviate all these changes(P<0.01or 0.05),while cilostazol only lowered blood CP and CRP levels(P<0.01).The level of the expression of iNOS mRNA was obviously higher in diabetic rats(P<0.01),but DBD and cilostazol could decrease it (both P<0.01).Conclusion:DBD may alleviate the disorder of blood glucose and lipid,lowerthe levels of GHb,CP and CRP,inhibit the expression of iNOS mRNA.These effects may slow down the progress of diabetic foot.