盐酸戊乙奎醚对创伤性急性肺损伤大鼠促炎性细胞因子的影响

目的:探讨盐酸戊乙奎醚(长托宁)对创伤性急性肺损伤(ALI)炎症反应影响及可能的机制。方法:建立创伤性急性肺损伤大鼠模型。实验动物随机分为正常对照(C)组、创伤模型(M)组、长托宁干预(P)组,每组24只。各组动物进行动脉血气、肺湿/干质量(W/D)比值、肺组织髓过氧化物酶活性(MPO)、血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平、光镜和电镜下观察肺组织损伤情况比较。结果:长托宁治疗后能不同程度降低TNF-α和IL-6的含量(P<0.01,P<0.05),明显改善创伤性急性肺损伤动物的血气(P<0.05),减少肺W/D(P<0.05)和MPO值(P<0.05),减轻肺组织的损伤程度。结论:促炎性细胞因子在创伤性ALI的发生、发展过程中起着重要作用;长托宁发挥抗炎作用与其对炎性细胞因子的调节作用密切相关。

Effects of Penehyclidine Hydrochloride on Traumatic Acute Lung Injury-Induced Proinflammatory Cytokine Production in Rats

Objective: To study the effects of Penehyclidine hydrochloride on the inflammatory reaction and their potential treatment mechanism in traumatic acute lung injury(ALI).Methods: Rat models of traumatic ALI were used in this study,and were randomly divided into normal control group(C),trauma model group(M) and Penehyclidine hydrochloride intervention group(P),with 24 rats in each group.The effects of penehyclidine hydrochloride on arterial blood gas,lung wet/dry(W/D) ratio,lung myeloperoxidase(MPO) activity,serum tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) level were observed.Light and electron microscopy were also performed to detect the injury of lung tissue.Results: Penehyclidine hydrochloride could significantly improve the blood gas results(P<0.05),reduce the lung W/D weight ratio(P<0.05) and MPO activity(P<0.05),decrease the plasma level of TNF-α and IL-6(P<0.01,P<0.05),and relieve the lung tissue injury in the traumatic ALI rats.Conclusion: Penehyclidine hydrochloride plays anti-inflammatory and protective roles in traumatic ALI through mediating the proinflammatory cytokines.