心力衰竭后心房瞬时受体电位通道的变化

目的:研究心力衰竭对心房组织瞬时受体电位通道C1(TRPC1)基因表达及其蛋白水平的影响。方法:新西兰白兔20只随机分为2组:对照组10只(其中假手术5只),心力衰竭组10只。采用主动脉瓣返流联合腹主动脉缩窄的方法制作慢性心力衰竭模型,10周后超声心动图检测心功能指标,应用RT-PCR和Western blot技术检测左心耳TRPC1基因表达和蛋白水平的变化。结果:心力衰竭组左房内径、室间隔厚度、左室后壁厚度、左室收缩和舒张末内径均明显增加(P<0.05),射血分数及左室短轴缩短率显著降低(P<0.05);与对照组相比,心力衰竭组左心耳TRPC1基因表达明显上升(P<0.05),TRPC1蛋白含量也显著高于对照组(P<0.05)。结论:兔心力衰竭后心房组织的TRPC1基因表达及其蛋白水平均明显升高,可能与心力衰竭后房性心律失常的发生有关。

Changes of Transient Receptor Potential Channels in Atrial Myocardium of Rabbits with Heart Failure

Objective:To observe the changes of gene expression and protein level of transient receptor potential channel(TRPC1)in isolated atrial myocardium after heart failure in rabbits.Methods:Twenty rabbits were randomly divided into two groups:control group(n=10,including five sham-operation rabbits) and heart failure(n=10)group.Chronic heart failure model was produced by combined aortic regurgitation and coarctation of the abdominal aorta.Cardiac structure and function were detected via echocardiography 10weeks later.The mRNA expression and protein level of TRPC1of left atrial appendage were detected by RT-PCR and Western blotting,respectively.Results:The left atria diameter,inter-ventricular septum,left ventricular post-wall dimension,left ventricular end diastolic and systolic diameter were also increased markedly in heart failure rabbits,but their ejection fraction and left ventricular shorten fraction were decreased(P<0.05).Compared with the control group,the expression of TRPC1 mRNA and protein in heart failure group were markedly increased(P<0.05).Conclusion:The mRNA and protein expression of TRPC1in atrial myocardia of rabbits with heart failure was significantly increased.TRPC1may play a role in the vulnerability to atrial arrhythmias in dilated atria with heart failure.