改良型非创伤性股骨头缺血坏死动物模型的建立

目的:探讨改良液氮冷冻法制作非创伤性股骨头缺血坏死(ANFH)的可行性,寻找一种较为理想的ANFH的造模方法。方法:将20只新西兰大白兔利用改良液氮冷冻法,制作ANFH模型,分别在术后2,4,6,8周行大体观、MRI、X线及Masson三色染色观察。结果:造模后2周股骨头变化不明显;造模后4周股骨头表面软骨变性,软骨下骨部分坏死;造模后6周股骨头表面软骨部分剥脱,软骨下骨坏死增多;造模后8周股骨头大部分骨质坏死,甚至出现塌陷。结论:改良液氮冷冻方法制作的股骨头坏死模型,其影像学及组织学改变较为符合早中期股骨头坏死的改变,且此法制作模型简单,费用低,坏死表现早,成功率高。

Establishment of a Modified Model of Non-Traumatic Avascular Necrosis of Femoral Head in Rabbits

Objective:To explore the feasibility of developing non-traumatic avascular necrosis of femoral head(ANFH) by improved liquid nitrogen freezing method,and to find a better way to establish the model of ANFH.Methods:Twenty New Zealand white rabbit models of ANFH were established by improved liquid nitrogen frozen method.The rabbits were examined through gross observation,MRI,X-ray film,and Masson staining at the end of the 2nd,4th,6th and 8th weeks post-operation respectively.Results:Models of the femoral head had no significant changes during the first two weeks post-operation;the surface cartilage of the femoral head degenerated and the subchondral bone showed necrosis partly after four weeks;the surface femoral head cartilage was partly stripped and the subchondral bone necrosis was severe after six weeks;the majority of femoral head became necrosis and the subchondral bone was even collapsed after eight weeks.Conclusion:The rabbit models of ANFH are established successfully by modified method of liquid nitrogen freezing,the images and histological changes are more consistent with the early and mid-term changes in femoral head.The method is simple and inexpensive,the necrosis appears early and the successful rate is high.