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| 髓样细胞分化蛋白抑制乙型肝炎病毒复制的机制研究 | |
| 引用本文： | 姚玉荣,刘宁,汪运山. 髓样细胞分化蛋白抑制乙型肝炎病毒复制的机制研究[J]. 济宁医学院学报, 2010, 33(4): 232-234,237. DOI: 10.3969/j.issn.1000-9760.2010.04.002 |
| 作者姓名： | 姚玉荣 刘宁 汪运山 |
| 作者单位： | 1. 蓬莱市人民医院,山东,蓬莱,265600;山东医学科学院,山东,济南,250062 2. 济宁医学院科研处,山东,济宁,272013 3. 山东医学科学院,山东,济南,250062 |
| 摘 要： | 目的探讨髓样细胞分化蛋白（MyD88）对乙型肝炎病毒复制的抑制作用及其机制。方法以连接了MyD88全长序列的腺病毒为载体感染HepG2.215细胞,免疫荧光法确认Ad5-MyD88的感染效率后,以无关蛋白GFP作为对照,用ELISA、Real-time PCR、Northern blotting、Southern blotting分别检测上清中的HBsAg和HBeAg的含量及HBV RNA、DNA的表达观察MyD88对HBV复制抑制作用的影响。结果与GFP相比,感染了带有MyD88序列腺病毒的HepG2.215细胞上清中HBsAg、HBeAg含量、细胞内RNA及Core particle DNA的水平全面下降。结论 MyD88可降低HepG2.215细胞内HBV的复制水平;MyD88在抑制HBV复制过程中有着一定的作用。 |
| 关 键 词： | 乙型肝炎病毒 髓样细胞分化蛋白 |
Molecular mechanisms of inhibition of MyD 88 protein to HBV gene replication |
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| YAO Yu-rong,LIU Ning,WANG Yun-shan. Molecular mechanisms of inhibition of MyD 88 protein to HBV gene replication[J]. Jounal of Jinning Medical College, 2010, 33(4): 232-234,237. DOI: 10.3969/j.issn.1000-9760.2010.04.002 | |
| Authors: | YAO Yu-rong LIU Ning WANG Yun-shan |
| Affiliation: | YAO Yu-rong,LIU Ning,WANG Yun-shan (Penglai People's Hospital,Yantai 265600,China) |
| Abstract: | Objective To investigate the molecular mechanisms of MyD88 protein inhibiting HBV gene replication.Methods The adenovirus containing the MyD88 sequence is used to infect HepG2.215 cells,and immunofluorescence employed to confirm the efficiency infection of Ad5-MyD88.Adenovirus-GFP as negative control,after infection 48h,the HBV RNA,HBsAg and HBeAg were detected respectively by ELISA,Real-time PCR,Northern blotting and Southern blotting.The HBV core particle DNA also was detected via real-time PCR.All the re... |
| Keywords: | Hepatitis B virus Myeloid differential primary response protein |
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