原癌基因N33在增生性瘢痕中表达的研究

目的探讨原癌基因N33在增生性瘢痕的形成与发展中基因分布特点与变化趋势,以及在增生性瘢痕中所起的作用及意义。方法收集增生性瘢痕患者24例、非增生性瘢痕患者24例不同时期的标本,按伤后3、6、9、12个月分组编号。另设12例患者的正常皮肤作为对照组。利用原癌基因N33的基因特异片段制成寡核苷酸探针,与瘢痕组织切片和成纤维细胞爬片原位杂交,经统计学处理后,分析其变化规律。结果早期增生性瘢痕中,N33基因的探针在组织细胞中有较强的阳性反应,而非增生瘢痕则阳性反应较低,正常皮肤组的阳性反应更少。经对所有标本的观察,增生性瘢痕3~6个月的N33表达,阳性细胞的数量、反应的着色程度,均明显强于9~12个月增生性瘢痕、非增生性瘢痕与正常皮肤组。将增生性瘢痕组与非增生性瘢痕组比较,两者差异有极显著意义。成纤维细胞爬片杂交结果与组织切片存在相同的结果。结论瘢痕增生与细胞原癌基因及抑癌相关基因之间存在密切关系,而原癌基因N33起到十分重要的作用。同时,调节该基因的表达,有望减轻瘢痕的增生。

Effects of gene expression of fibroblast proto-oncogene N33 on the hypertrophic scar formation

Objective To investigate the effect of gene expression of fibroblast proto-oncogene N33 on the formation and contraction of hypertrophic scar. Methods Selected segments of N33 cDNA were usded to made an oligonucleotide probe. 24 cases with hypertrophic scar and 24 with non-hypertrophic scar were collected for the experimental groups and they were assorted according to the length of the time following burn injury (3, 6, 9, 12 months). 12 cases with the normal skins were usedd as the control group. Freezntissue sections and cultured fibroblasts were used to detect the expression of these genes by hybridization in situ. Results The expression of N33 was found in scar tissue, but expression in hypertrophic scar was significantly stronger than in non-hypertrophic scar. Conclusion Over-expression of proto-oncogene N33 may have an important functions on the formation of hypertrophic scar.