鼠晶状体上皮细胞的体外培养

目的:建立鼠晶状体上皮细胞体外培养的模型。方法:应用组织块贴片法和酶逐步消化法对10~14d的SD大鼠晶状体上皮细胞进行体外培养,在相差显微镜下观察其生长规律。结果:组织块贴片法在加入培养基4~5d后见细胞生长,2wk细胞融合。而酶逐步消化法在加入培养基后7d左右见细胞贴壁,2wk左右见细胞融合。结论:鼠晶状体上皮细胞体外培养较困难,本试验采用酶逐步消化方法和组织块贴片法。成功地建立了鼠晶状体上皮细胞体外培养的模型,为研究后发性白内障发病机制提供了基础。

Culture system in vitro of rat cataract leas epithelial cells

AIM:To establish a culture system in vitro of rat cataract lens epithelial cells.· METHODS:Cataract lens epithelial cells of postnatal 10-14 days SD rat were cultured by tissue block adherence and gradual enzymatic digestion.Cultured cells growth was observed by phase-contrast microscopy.· RESULTS:In the culture of rat lens epithelial cells by tissue block explant,cells attached and started growing from the 4th-5th day,and cell fusion occurred during 2 weeks.In the culture by enzymatic digestion,cells didn’t attach until the 7th day,while cell fusion was found in 2 weeks.· CONCLUSION:Rat cataract lens epithelial cells are delicate and difficult to be cultured.We have successfully set up the culture system of rat lens epithelial cells,which might provide a methodological foundation for research on pathogenesis of posterior capsule opacification.