SEB双单克隆抗体免疫检测试剂盒研制 |
| |
引用本文: | 梁秀敏,张靖,杨廷松,刘威,李艳军,李长平. SEB双单克隆抗体免疫检测试剂盒研制[J]. 卫生研究, 1998, 0(Z1) |
| |
作者姓名: | 梁秀敏 张靖 杨廷松 刘威 李艳军 李长平 |
| |
作者单位: | 北京防化研究院 |
| |
摘 要: | 为了确定食品有否SEB污染,我们以单抗1D2为包被抗体,2D1为酶标抗体,42℃5小时为包被条件,研制了SEB双单克隆抗体快速免疫检测试剂盒。在SEB浓度为0.1~30μg/L的范围内,浓度和测量的A值呈直线相关,相关系数R=0.9786,a=-9.8945,b=34.2477,检测灵敏度为0.1μg/L,人工样品回收率在95%以上。该试剂盒具有快速、灵敏、准确的特点,适合医院、食品卫生检验单位使用
|
关 键 词: | 金黄色葡萄球菌肠毒素B(SEB) 单克隆抗体 免疫检测试剂盒 |
Double Monoclonal Antibodies Immunoassay Kit for Detection SEB |
| |
Abstract: | おhe immunoassay Kit for double monoclonal antibodies(McAb) against staphylococcal enterotoxin B (SEB) was established.40 well microtest plates were coated with 1D2 McAb 42℃ 5 hours,McAb 2D1 was labled with horseradish peroxidese (HRP).The concentration (C) of SEB in sample varied from 01 to 30 μg/L,there was line correlation between C and A measured.The correlation coefficients R=09786,a=-98945,b=342477.Detection limit was 01μg/L.The recovery of SEB in contaminated food by the atifical was above 95%.The Kit for the detection SEB was rapid,sensitive and accuracy,it may use for the hospital,test department for food hygiene. |
| |
Keywords: | monoclonal antibody staphylococcal enterotoxin B immuonassay Kit |
本文献已被 CNKI 等数据库收录! |
|