胃癌细胞 p27kip1 、cyclinE 的表达及与幽门螺杆菌感染关系

目的]观察胃癌细胞p27^kjp1、cyclinE的表达情况，并分析幽门螺杆菌（Heticobacter pylori，Hp）感染对二者表达的影响，以探讨Hp可能的致癌机制。方法]用免疫组织化学SP法检测59例胃癌p27^kip1、cyclinE表达情况，采用改良Giemsa快速尿素酶检验法检测HD感染。结果]p27hp。阳性率在正常胃黏膜为84．22％，显著高于胃癌的47．46％（P〈0．05），胃癌中高中分化腺癌组为68．42％，显著高于低分化腺癌的37．5％和与胃黏液癌的31．25％（P〈0．05），但后两者间无显著性差异（P〉0．05）。cyclinE阳性率在正常胃粘膜为11．1l％，显著低于胃癌的55．93％（P〈0．05），胃癌中高中分化腺癌组为31．58％显著低于低分化腺癌的66．67％和胃黏液癌的68．75％（P〈0．05），后两者间无显著性差异（P〉0．05）。p27kjP0及cyclinE的阳性率在HD阳性和HD阴性胃癌组间无显著差异性（P〉0．05）。结论]胃癌组织p27kipl表达下调、cyclinE表达上调，提示二者的表达失衡可能会促进胃癌的发生。但二者表达与HD感染无明显相关关系。

Expressions of p27kip1 and cyclinE in gastric cancer and their relationship to helicobacter pylori infection

Objective] To investigate p27~(kip1) and cyclinE gene product expressions of carcinoma cells and the relationship of the expressions to Helicobacter pylori(Hp) infection in gastric carcinoma.Methods] The expressions of p27~(kip1) and cyclinE in 59 gastric carcinomas were detected by immunohistochemical method(SP method) and Hp was examined by Giemsa staining and rapid urease test.Results] The p27~(kip1) expression rate of normal gastric mucosa being 84.22% was significantly higher than that of gastric carcinoma being 47.46%(P<(0.05);) the rate of well-middle differentiative carcinomas being 68.42% was significantly higher than those of poor differentiative carcinoma being 37.50% and mucoid carcinoma being 31.25%(P<0.05).The cyclinE expression rate of normal gastric mucosa being 11.11% was significantly lower than that of gastric carcinoma being 55.93%(P<(0.05);) the rate of well-middle differentiative carcinomas being 31.58% was significantly lower than those of poor differentiative carcinoma being 66.67% and mucoid carcinoma being 68.75%(P<(0.05).)In gastric carcinoma,there was no significant relationship of the expressions of p27~(kip1) and cyclinE to Hp infection(P>0.05).Conclusion] The lower expression of p27~(kip1) and the higher expression of cyclinE are associated with the development and the progression of gastric cancer,and there was no singificant association of the expression of p27~(kip1)/cyclinE with Hp infection.