三氯乙烯对豚鼠皮肤致敏作用与肝肾功能的损害

目的 探讨三氯乙烯(TCE)对豚鼠皮肤致敏作用及肝肾功能的损害.方法 采用豚鼠最大值试验(GPMT),将动物分成阴性对照组、阳性对照组和TCE实验组,每组6只豚鼠,分别皮内注射橄榄油、2,4-二硝基氯苯(DNCB)和TCE.实验结束后观察动物皮肤改变,应用自动生化分析仪检测致敏动物血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、白蛋白、球蛋白、乳酸脱氧酶(LDH)、肌苷、尿酸等指标.结果阳性对照组和TCE实验组动物出现明显皮肤红斑、水肿,阳性对照组动物致敏率为100%,TCE实验组动物致敏率为83.3%.阳性对照组动物血清中ALT、AST活力升高,TCE实验组动物血清中ALT、AST、LDH活力明显高于阴性对照组,差异有统计学意义(P＜0.05或P＜0.01).结论 TCE可诱导豚鼠产生明显的皮肤致敏作用,属强致敏物,并可引起实验动物肝功能指标的改变.

Skin sensitization as well as liver and kidney impairment in guinea pigs treated with trichloroethylene

Objective To study skin sensitization as well as liver and kidney impairment in guinea pigs treated with trichloroethylene (TCE).Methods Guinea pig maximization test (GPMT) was applied in this study.Guinea pigs were divided into 3 groups,namely negative control,positive control and TCE treatment.Animals of 3 groups were administrated with olive oil,2,4-dinitrochlorobenzene (DNCB),and TCE,respectively,by intradermal injection.The animal skin was observed and blood was collected after various treatment.The liver function tests were conducted,including detection of activities of ALT,AST,LDH and levels of creatinine,uric acid,and urea with automatic biochemical analyzer.Results Obvious skin impairment was observed in the groups of positive control and TCE treatment.The skin impairment included erythema and edema.The sensitization rate was 100% in positive control and 83.3% in TCE treatment group.Additionally,the activities of ALT,AST and LDH increased significantly in the groups of positive control and TCE treatment when compared with the negative control (P＜0.05 or P＜0.01 ).Conclusions Trichloroethylene is one of the strong hypersensitizing substances,and it could induce skin allergic reaction and liver impairment in guinea pigs.