丹参多酚酸盐体外抗肿瘤作用研究

目的:研究丹参多酚酸盐体外抗肿瘤的作用,探讨其可能机制。方法:采用MTT比色法测定注射用丹参多酚酸盐体外对人肝癌细胞株SMMC-7721细胞、人肺腺癌细胞株SPC-A-1细胞、B淋巴瘤细胞株Raji细胞和急性粒细胞白血病细胞株HL-60细胞4种肿瘤细胞株增殖的影响;采用流式细胞仪检测细胞周期的改变及细胞凋亡情况;采用吉姆萨染色及吖啶橙荧光染色观察细胞形态变化。结果:注射用丹参多酚酸盐对SMMC-7721、SPC-A-1、Raji、HL-60细胞增殖均有显著的抑制作用,且呈时间和剂量依赖关系;可诱导SMMC-7721细胞阻滞于S期继而发生细胞凋亡;可观察到细胞逐渐凋亡形态。结论:注射用丹参多酚酸盐有较强的体外抗肿瘤作用,可能是通过作用于细胞周期、诱导肿瘤细胞凋亡来发挥的。

Anti-tumor Activity and Its Mechanism of Depsides Salts from Salvia miltiorrhiza in Vitro

OBJECTIVE:To study the in vitro anti-tumor activity of Depsides salts from Salvia miltiorrhiza and its possible mechanism.METHODS:The effects of Depsides salts from Salvia miltiorrhiza on cell proliferation in four human tumor cell lines including SMMC-7721,SPC-A-1,Raji,and HL-60 in vitro was tested by MTT.Cell cycle distribution and apoptosis were detected by flow cytometry.Giemsa staining and acridine orange(AO) fluorescence staining were used to observe the morphological changes of cells.RESULTS:Depsides salts from Salvia miltiorrhiza inhibited the proliferation of Raji,HL-60,SPC-A-1 and SMMC-7721 cells obviously in a dose-and time-dependent manner and arrested SMMC-7721 cells at Sphase which then led to apoptosis.Typical morphological changes of apoptosis were also observed in Depsides salts from Salvia miltiorrhiza-treated cells.CONCLUSION:The remarkable anti-tumor activity in vitro of the Depsides salts from Salvia miltiorrhiza is possibly achieved by blocking cell cycle and then inducing apoptosis of tumor cells.