Synthesis and evaluation of cis-1-[4-(hydroxymethyl)-2-cyclopenten-1-yl]-5-[(124)I]iodouracil: a new potential PET imaging agent for HSV1-tk expression |
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Authors: | Ahn Hyunseok Choi Tae Hyun De Castro Kathlia Lee Kyo Chul Kim Byoungsoo Moon Byung Seok Hong Su Hee Lee Jong Chan Chun Kwon Soo Cheon Gi Jeong Lim Sang Moo An Gwang Il Rhee Hakjune |
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Institution: | Hanyang University, Department of Chemistry and Applied Chemistry, Ansan, Sangrok-Gu, Sa-3-Dong 1271, Kyunggi-Do 426-791, Korea. |
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Abstract: | In our pursuit to find an appropriate reporter probe for herpes simplex virus type-1 thymidine kinase (HSV1-tk), a carbocyclic nucleoside analogue, cis-1-4-(hydroxymethyl)-2-cyclopenten-1-yl]-5-124I]iodouracil, has been efficiently synthesized. A Pd(0)-catalyzed coupling reaction together with organotin and exchange reactions for radiolabeling gave more than 80% radiochemical yield with greater than 95% radiochemical purity and 1.15 GBq/mumol specific activity. Biological data reveal that the analogue is stable in vitro, less toxic than ganciclovir (GCV), and selective to HSV1-tk-expressed cells based on micro positron emission tomography (microPET) image analyses. Thus, this new carbocyclic nucleoside, referred to in this paper as carbocyclic 2',3'-didehydro-2',3'-dideoxy-5-iodouridine (carbocyclic d4IU) is a potential imaging probe for HSV1-tk. |
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