组织芯片技术检测人睾丸基因TDRG1在睾丸肿瘤组织中的表达

目的:探讨人睾丸基因TDRG1在睾丸肿瘤组织中的蛋白表达及其病理学意义。方法:运用组织芯片技术、免疫组化法检测人睾丸特异基因TDRG1在睾丸肿瘤组织和正常睾丸组织中的表达。结果:15例正常人睾丸对照组中11例(73.3%)TDRG1蛋白表达为阳性;26例精原细胞瘤中7例(26.9%)TDRG1蛋白表达为阳性,7例畸胎瘤中4例(57.1%)TDRG1蛋白表达为阳性。而12例胚胎癌中10例(83.3%)TDRG1蛋白表达为阳性,10例卵黄囊瘤中8例(80.0%)TDRG1蛋白表达为阳性。精原细胞瘤实验组与正常人睾丸对照组相比较,两组间具有极显著性差异(P<0.01)。畸胎瘤实验组与正常人睾丸对照组相比较,两组间具有显著性差异(P<0.05)。而胚胎癌组和卵黄囊瘤组与正常人睾丸对照组相比较,没有显著性差异(P>0.05)。结论:TDRG1蛋白在精原细胞瘤和畸胎瘤的表达水平较正常对照睾丸组织显著降低,TDRG1可能为候选的抑癌基因。

Expression of human testis development related gene 1 in testicular cancer detected by tissue microarray

Objective:To investigate the protein expression of human testis development related gene 1 (TDRG1) in human testicular cancer and its pathological significance. Methods:The expression levels of TDRG1 were detected in the testis tissues of testicular cancer patients and normal men by tissue microarray and immunohistochemistry,and the results were analyzed. Results:Immunohistochemistry exhibited positive expression of the TDRG1 protein in the testis of 73.3% (11/15) of the normal men,83.3% (10/12) of the patients with embryonal carcinoma,80.0% (8/10) of those with yolk sac tumor,26.9% (7/26) of those with seminoma,and 57.1% (4/7) of those with teratoma. The expression levels of TDRG1 in the testis tissues of the seminoma and teratoma groups were shown to be significantly lower than that of the normal control (P < 0.01 and P < 0.05),but those of the embryonal carcinoma and yolk sac tumor groups exhibited no significant differences from that of the latter (P > 0.05). Conclusion:The significantly reduced expression of the TDRG1 protein in patients with seminoma or teratoma indicates that TDRG1 may be a candidate cancer suppressor gene.