RAD51基因在结直肠癌中的表达及与预后的关系

目的 探讨RAD51基因在结直肠癌（CRC）中的表达及其与临床病理特征和预后的关系。方法 收集72例CRC患者的病理标本（CRC组，n=72），以及经病理证实无癌细胞存在、距肿瘤边缘>3 cm的肠黏膜组织（癌旁对照组，n=72）。采用qRT-PCR和Western blotting技术对CRC与癌旁组织的RAD51 mRNA和蛋白表达水平进行检测。影响CRC患者预后的因素采用Cox比例风险模型分析。预后分析采用Kaplan-Meier和Log-rank法。结果 CRC组RAD51 mRNA和蛋白相对表达量均高于癌旁对照组，差异有统计学意义（P<0.05）。CRC组织中RAD51表达水平与TNM分期有关（P<0.05）。RAD51高表达是影响CRC患者5年无瘤生存率和5年总体生存率的危险因素（P<0.05）。RAD51低表达的CRC患者5年总体生存率和5年无瘤生存率均高于RAD51高表达患者（P<0.05）。结论 RAD51在CRC组织中高表达，与TNM分期有关。RAD51高表达的CRC患者预后差，RAD51可能是临床上预测CRC患者预后的一个潜在指标。

Expression of RAD51 gene in colorectal cancer and its relationship with prognosis

Objective To explore the expression of RAD51 gene in colorectal cancer (CRC) and its relationship with clinicopathological features and prognosis.Methods The pathological specimens of 72 CRC patients were collected (CRC group, n=72), as well as the normal intestinal mucosa which was pathologically conformed with no tumor cells and was >3 cm away from the edge of the tumor (control group, n=72). The expression levels of RAD51 mRNA and protein in CRC and adjacent tissues were detected by qRT-PCR and Western blotting. The prognostic factors of CRC patients were analyzed by Cox proportional hazards model. The prognosis was analyzed by Kaplan-Meier and Log-rank methods.Results The relative expression levels of RAD51 mRNA and protein were higher in CRC group than in control group (P<0.05). The expression level of RAD51 in CRC was correlated with TNM stage (P<0.05). The high expression of RAD51 was an independent factor affecting 5-year overall survival rate and 5-year tumor-free survival rate of CRC patients (P<0.05). The 5-year overall survival rate and 5-year tumor-free survival rate of patients with low expression of RAD51 in CRC tissues were higher than those in patients with high expression of RAD51 (P<0.05).Conclusion RAD51 was highly expressed in CRC tissues, and it was related with TNM staging. CRC patients with high expression of RAD51 in tissues had poor prognosis. RAD51 may be a potential prognostic indicator for CRC patients.