Magnitude and genotype of hepatitis delta virus among chronic hepatitis B carriers with a spectrum of liver diseases in Ethiopia |
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Institution: | 1. Department of Microbiology, Immunology, and Parasitology, School of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia;2. Institute of Virology, University of Leipzig, 04103 Leipzig, Germany;3. Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia;4. School of Medicine, College of Medicine and Health Sciences, Hawassa University, Hawassa, Ethiopia;5. School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia;6. Yanet Specialized Clinic, Hawassa, Ethiopia;7. Department of Internal Medicine, School of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia;8. Adera Medical Center PLC, Addis Ababa, Ethiopia;9. Department of Internal Medicine, St. Paul''s Hospital Millennium Medical College, Addis Ababa, Ethiopia |
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Abstract: | Introduction and ObjectivesChronic hepatitis D infection contributes substantially to the progression of chronic liver disease, especially in most low and middle-income countries, where hepatitis B virus-related chronic liver disease is endemic. Therefore, this study aimed to determine the magnitude and genotype of hepatitis delta virus (HDV) among patients with chronic hepatitis B (CHB)-related liver diseases in Ethiopia.Patients and MethodsIn this cross-sectional study, 323 known HBsAg positive individuals comprising 220 patients with CHB-related liver diseases 121 advanced liver diseases (hepatocellular carcinoma /HCC/ and non-HCC) and 99 chronic hepatitis (CH)], and 103 symptomless blood donors (BD) were enrolled. An ELISA kit was employed to determine HDV infection, and quantitative real-time PCR was used to detect HDV RNA. In addition, a non-coding genomic RNA region was sequenced for genotyping and phylogenetic analysis.ResultsIrrespective of the stage of liver disease, the overall magnitude of HDV was 7.7% (25/323). The frequency of anti-HDV increases with the severity of liver disease, 1.9%, 4%, 10%, and 21.3% among BD, CH, non-HCC, and HCC patients, respectively. HDV RNA has been detected in 1.54 %(5/323) cases with a mean viral load of 4,010,360 IU/ml. All isolates were found to be HDV genotype 1.ConclusionsThe magnitude of HDV infection increased with the severity of liver disease, indicating HDV infection is more common among patients with CHB-related liver diseases in Ethiopia. |
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