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CpG island methylator phenotype of multigene in serum of sporadic breast carcinoma |
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| Authors: | Feng Jing Wang Yuping Chen Yong Luo Jie Lu Jun Tang Xuanbing Hu Lihua |
| Affiliation: | 1. Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China 2. Southern Medical University, Fengxian Hospital, Shanghai, China 3. Taihe Hospital, Yunyang Medical College, Shiyan, China |
| Abstract: | CpG island methylator phenotype (CIMP) involves methylation targeted toward the promoters of multiple genes. We determined a methylation profile of tumor-related genes in serum of sporadic breast cancer (SBC). The multigene methylation was examined by methylation-specific polymerase chain reaction assay in serum of 50 SBCs and 50 paired nontumors, and CIMP+ was defined as having three genes that are concordantly methylated. The methylation frequency of ten genes in serum of 50 SBCs varied from 10% in FHIT to 74% in RASSF1A. The methylation status of RASSF1A, BRCA1, p16, CDH1, ER, RARβ2, APC, and DAPK was significantly correlated with SBC and nontumor serum (P?P?RASSF1A, BRCA1, p16, CDH1, ER, RARβ2, APC, and DAPK (P?CDH1, RASSF1A, BRCA1, and RARβ2 as breast cancer marker may contribute high sensitivity (90%) and specificity (88%). ER and RARβ2 methylation was associated with elevated serum CA153 levels in 39 SBC samples with CIMP+ (P? |
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