间接免疫荧光法及蛋白质印迹法对肺癌患者体内自身抗体的分析

目的：了解肺癌患者血清自身抗体阳性率、细胞内定位及荧光图形特点，探索自身抗体作为肿瘤樗物的可能性。方法：应用间接免疫荧光法（ｉｎｄｉｒｅｃｔ ｉｍｍｕｎｏｆｌｕｏｒｅｓｓｅｅｎｅｅ，ＩＩＦ）检测７７份肺癌以及１４０份不同年龄段正常人血清中自身抗体。提取人喉癌上皮细胞（Ｈｅｐ－２）蛋白抗原，采用蛋白质印迹法对８１份肺癌患者及５２份正常人血清进行分析。结果：肺癌组和对照组的自身抗体阳性率有显著差别，两

Analysis of autoantibodies in lung cancer patients with IIF and Western blotting

Objective: To study the positive rate of autoantibodies, the locations of their target antigens in cells and the essence of their target antigens in lung cancer patients. Methods: With indirect immunofluorescence(IIF),sera of 77 lung cancer patients and 140 healthy controls were detected. Using antigens extracted from human epithelial cells (Hep-2),we detected 81 cases of lung cancer and 52 cases of healthy controls with Western blotting. Results: The positive rate of autoantibodies in lung cancer group and normal group was 57. 14% and 17. 14%, respectiviy. Although both groups had higher positive rates with the growth of age (P<0. 05), cancer group significantly higher than that of normal group (P<0. 01). The autoantibody IIF photoes, intracellulay location of target antibody were obviously different in cancer group and control group. In western- blotting test, one or more positive bands were found in 74 lung cancer patients (91. 4 %, 74/81 ), which was significantly higher than normal group (31/52, 61. 5 % ) (P<0. 01 ). Much of them could not be explained by the known autoantibodies appear- ing in autoimmune diseases. A common positive band with related molecular mass about 65 000 was found in 67 patients (82. 7% ), while in 22. 2% normal group (12/52). Conclusion: Autoantibodies in lung cancer are different from those in normal controls and patients with autoimmune disease. Further efforts should be made to study the target antigens and their biological role and clinical significance.