蜂毒多肽长循环脂质体的制备研究

 目的研制蜂毒多肽长循环脂质体。方法采用薄膜超声分散法制备蜂毒多肽长循环脂质体,以包封率为指标,分别考察药脂比、胆固醇用量、磷脂种类、不同相对分子质量PEG-胆固醇、不同pH值、离子强度等对脂质体的影响,在此基础上采用正交设计[L₉(4³)]对处方进行优化。结果制得的长循环脂质体包封率为(86.13±0.51)%,平均粒径为(74.43±5.53)nm,4℃放置10d包封率无变化。结论蜂毒多肽长循环脂质体包封率、稳定性较高,且制备工艺简单、重现性好。

Preparation of sterically stablized nanoliposomes of bee venom peptide

OBJECTIVE To prepare the sterically stabilized nanoliposomes.METHODS The sterically stabilized nanoliposomes were prepared with membrane-sonic method, the effect of some factors on the encapsulation efficiency, including lecithins, the weight ratio of bee venom to SPC, the weight ratio of SPC to chol,the pH of water phase, the electricity of lipids and the iron strength of water phase, was investigated. Then, the formulation was optimized by orthogonal design.RESULTS The encapsulation efficiency of the liposomes was (86.13±0.51)%, and there was no change within 10 d.The average diameter of the liposomes was (74.43±5.53)nm.CONCLUSION The liposomes were prepared with good encapsulation efficiency and stability, and the preparation was simple, feasible and replicable.