| RP-HLPC法同时测定藿香正气水中6种有效成分的含量 |
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| 引用本文： | 蔡洪鲲,刘小芬,蒋范任,高洪琳,孙国祥.RP-HLPC法同时测定藿香正气水中6种有效成分的含量[J].中南药学,2014(6):577-581. |
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| 作者姓名： | 蔡洪鲲 刘小芬 蒋范任 高洪琳 孙国祥 |
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| 作者单位： | [1]湖南省益阳市食品药品检验所,湖南益阳413000; [2]益阳医学高等专科学校,湖南益阳413000; [3]沈阳药科大学药学院,沈阳110016 |
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| 摘 要： | 目的建立反相高效液相色谱法同时测定藿香正气水中4种药材的6种有效成分的含量。方法采用反相高效液相色谱法,C18色谱柱(Gemini NX,250 mm×4.6 mm,5μm),以0.05%甲酸(A)-乙腈(B)为流动相进行梯度洗脱,流速1.0 mL·min-1,检测波长270 nm。结果建立了同时检测藿香正气水中橙皮苷、甘草酸铵、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚6个成分的含量测定方法,阴性样品均无干扰,且样品分离好,无干扰,方法学验证合格。结论本方法快捷可行,检测限、灵敏度、线性关系、专属性等均符合含量分析要求,适合藿香正气水多指标组分的同时定量控制。
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| 关 键 词： | 藿香正气水 橙皮苷 甘草酸铵 欧前胡素 异欧前胡素 和厚朴酚 厚朴酚 |
Simultaneous determination of 6 effective components in Huoxiang Zhengqi liquid by RP-HPLC |
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| Institution: | CAI Hong-kun, LIU Xiao-fen, JIANG Fan-ren, GAO Hong-lin, SUN Guo-xiang (1. Yiyang Institute for Food and Drug Control, Yiyang Hunan 413000; 2. Yiyang Medical College, Yiyang Hunan 413000; 3. College of Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016) |
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| Abstract: | Objective To establish an RP-HPLC method to determine the content of 6 components in 4 medicinal herbs in Huoxiang Zhengqi liquid simultaneously. Methods The method was performed on a C18 chromatographic column （Gemini NX, 250 mm ×.6 mm, 5μm）, with 0.05% formic acid （A）-acetonitrile （B） as the mobile phase in gradient elution, the flow rate was 1.0 mL · min 1, and the detect wavelength was 270 nm. Results The RP-HPLC method for the determination of 6 ingredients in Huoxiang Zhengqi liquid was established, including hesperidin, glyc- yrrhizic acid, imperatorin, honokiol, isoimperatorin and magnolol. The negative samples were validated without inter- ferences, and separated well without impurity peaks. Conclusion The method is fast and feasible. The detection limit, specificity, sensitivity, and linearity verified and meet the requirements of content determination, appropriate for multi constituents in Huoxiang Zhengqi liquid and quality control. |
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| Keywords: | Huoxiang Zhengqi liquid hesperidin glycyrrhizic acid imperatorin isoimperatorin honokiol magnolol |
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