| 加味丹参饮高效液相色谱指纹图谱研究 |
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| 引用本文： | 童巧珍,黄政德,蔡嘉洛,张聪子,郭婷,孙梦姗. 加味丹参饮高效液相色谱指纹图谱研究[J]. 中南药学, 2014, 0(6): 504-506,517 |
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| 作者姓名： | 童巧珍 黄政德 蔡嘉洛 张聪子 郭婷 孙梦姗 |
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| 作者单位： | 湖南中医药大学,长沙410208 |
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| 基金项目： | 国家自然科学基金（No.81373576）; 湖南省中医药管理局重点项目（No.201304） |
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| 摘 要： | 目的建立加味丹参饮的HPLC指纹图谱。方法色谱柱:C18(4.6 mm×250 mm,5μm),流动相:甲醇-甲酸-乙腈梯度洗脱,检测波长为270 nm,流速1 mL·min-1,柱温30℃。结果确定了14个共有峰,10批加味丹参饮指纹图谱的相似度均>0.9。结论该方法简单、准确、重复性好,可为加味丹参饮的质量评价提供依据。
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| 关 键 词： | 加味丹参饮 高效液相色谱 指纹图谱 相似度 |
HPLC fingerprint of Jiawei Danshen decoction |
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| TONG Qiao-zhen,HUANG Zheng-de,CAI Jia-luo,ZHANG Cong-zi,GUO Ting,SUN Meng-shan. HPLC fingerprint of Jiawei Danshen decoction[J]. Central South Pharmacy, 2014, 0(6): 504-506,517 |
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| Authors: | TONG Qiao-zhen HUANG Zheng-de CAI Jia-luo ZHANG Cong-zi GUO Ting SUN Meng-shan |
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| Affiliation: | (Hunan University of Traditional Chinese Medicine, Changsha 410208) |
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| Abstract: | Objective To establish an HPLC fingerprint of Jiawei Danshen decoction. Methods Chromatographic column was C18 （4.6 mm× 250 ram, 5μm）, the mobile phase was acetonitrile-methanol-formic acid in a gradient elu- tion mode and the detection wavelength was 270 nm. The flow rate was 1 mL ·min- 1, and the column temperature was 30℃. Results Fourteen peaks were confirmed, and fingerprint similarity of 10 batches of Jiawei Danshen decoction was higher than 0.9. Conclusion The method is simple and accurate with a good reproducibility, and provides a basis for quality evaluation of Jiawei Danshen decoction. |
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| Keywords: | Jiawei Danshen decoction HPLC fingerprint chromatograrn similarity |
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