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| PCR技术在流脑监测中的应用研究 | |
| 引用本文： | 乔凤娟,任林柱,王会,王艳华,曹国春,高真真. PCR技术在流脑监测中的应用研究[J]. 中国卫生工程学, 2008, 7(4): 198-199 |
| 作者姓名： | 乔凤娟 任林柱 王会 王艳华 曹国春 高真真 |
| 作者单位： | 1. 长春市疾病预防控制中心,吉林,长春,130033 2. 吉林大学畜牧兽医学院 3. 吉林省疾病预防控制中心 |
| 摘 要： | 目的建立脑膜炎奈瑟菌种属及群的快速检出方法。方法应用PCR法扩增标准菌株及标本中脑膜炎奈瑟菌种属及各群的特异性DNA片段，通过电泳后对产物进行分析。结果11份标本中检出4份脑膜炎奈瑟菌CrgA基因片段，其中扩增出2份含有NmA群Off-2基因片段，2份含有NmC群siaD（C）片段。结论PCR技术可用于临床标本中脑膜炎奈瑟菌种属及各群的特异性检测。 |
| 关 键 词： | PCR DNA 流脑 |
Application of PCR Technology in Epidemic Cerebrospinal Meningitdis Surveillance |
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| Affiliation: | QIAO Feng -juan, REN Lin - zhu, WANG Hui, et al( Changchun Center for Disease Control and Prevention, Chuangchun Ji- lin 130033, China) |
| Abstract: | Objective To develop a rapid detection method for nesseria meningitidis species and group. Methods PCR method was used to amplify the standard strains of neisseria meningitidis bacteria in the samples and a group of specific DNA fragments by electrophoresis after an analysis of the product. Results 4 of 11 specimens were detected neisseria meningitidis CrgA gene fragments, which were amplified containing 2 NmA group Off - 2 gene fragments, 2 NmC group siaD （C） fragments. Conclusion The PCR technique can be applied to clinical specimens in the neisseria meningifidis bacteria and specific detection of DNA. |
| Keywords: | PCR DNA |
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