可移植兔原位高转移性膀胱癌模型的建立

目的 建立可移植高转移性兔原位膀胱癌模型。方法 VX－2细胞株采用肿块包埋法、细胞悬液注射法移植于新西兰兔膀胱。分别于1、2、3周后处死动物及5－6周荷瘤兔死亡后行病理检查移植瘤生长情况及淋巴结、肝、双肺转移情况。结果 两种方法接种成功率均为100％。1周后肿瘤大小为0．5cm,2周为1．0－2．0cm,3周后膀胱全程浸润,同时出现广泛淋巴结及双肺转移。细胞悬液注射法组兔的肿瘤生长及转移速度慢于肿块包埋法组。结论 VX－2细胞株制成家兔原位膀胱癌具有种植成功率高、高转移性特点,其生物学特性与高度恶性的膀胱肿瘤一致。

Establishment of transplantable and highly metastatic bladder neoplasms model in rabbits

Objective To establish a transplantable and highly metastatic bladder neoplasms model in rabbits.Methods VX 2 cells were implanted into New Zealand white rabbits bladder wall by mass embedded and cells injected method. The implanted tumors' situation of growth, infiltration and metastasis in lymph nodes,liver and lungs were analyzed grossly and histologically when the rabbits were sacrificed or died 1, 2, 3 and 5 6 weeks after surgery.Results The successful rate for both two different implanted methods was 100%. One, 2 weeks after surgery, the implanted tumors' diameter was 0.5 cm, 1.0 2.0 cm respectively. Three weeks after surgery, all layers of the bladder wall, extensive lymph nodes, liver and bilateral lungs were infiltrated. The growth and metastasis of the tumor in the cells injected group was slower than in the mass embedded group.Conclusion The established bladder neoplasms was easy to be implanted and highly metastatic. It has the same biological characters as high malignant bladder primary neoplasms.