枳椇云母汤对大鼠肝组织和胃黏膜乙醇脱氢酶活性及血乙醇浓度的影响

目的:观察大鼠急性酒精性肝损伤后肝组织和胃黏膜乙醇脱氢酶(ADH)活性及不同时间点血浆乙醇浓度的变化,探讨枳棋云母汤对急性酒精性肝病的保护作用.方法:60只SD大鼠随机分为正常组、模型组、枳椇云母组、云母组、枳椇子组和对照组(凯西莱组),每组10只.分别给予生理盐水、高度白酒、高度白酒+枳棋云母溶液、高度白酒+枳棋子溶液、高度白酒+云母溶液、高度白酒+凯西莱溶液灌胃.分别用比色法测定肝组织和胃黏膜组织ADH活性,采用顶空气相色谱法测定不同时间点血浆乙醇浓度变化.结果:枳椇云母汤能显著提高肝组织和胃黏膜组织ADH活性(P<0.05,P<0.01),降低血浆乙醇浓度(P<0.01).结论:枳犋云母汤有一定的护肝解酒作用,对酒精所引起的大鼠肝损伤具有较好的保护作用.

Effect of Zhijuyunmu Decoction on ADH Activity of Hepatic/stomach Tissue and Serum Ethanol Level in Rats

Abstact Object:To study the effects of Zhijuyunmu decoction on ADH activity of hepatic /stomach tissue and serum ethanol in rats with acute alcoholic hepatic damage.Methods :Sixty SD rats were divided into normal control group,model matched group,Zhijuyunmu-treated group,Yunmu-trated group,Zhijuzi-treatment group,and tiopronin group,10 in each.Each group was administered intragastically the corresponding solution.ADH activity was tested by the colorimetric method and serum ethanol level was determined by a gas chromatographic analysis.Results:Zhijuyunmu decoction increased the activity of ADH in hepatic/ stomach tissues and reduced the ethanol level in the sera(P<0.05 and P<0.01).Conclusion:Zhijuyunmu decoction has protective effect on acute alcohol hepatic tissue damage.