雷公藤多苷对佐剂性关节炎大鼠P 物质表达的影响

目的:探讨雷公藤多苷对佐剂性关节炎(AA)大鼠P物质(SP)的干预作用.方法:以AA大鼠为研究对象,以肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)作为炎症活动的指标,造模成功后,予雷公藤多苷片混悬液灌胃治疗30天,分别在治疗前后用放射免疫法测定各组大鼠血液、滑膜中的TNF-α、IL-1β、SP以及脊髓后角中的SP水平,以观察雷公藤多苷治疗后各组织SP的变化.结果:与正常组比较,模型组大鼠血液和滑膜中的SP、TNF-α、IL-1β和脊髓后角中的SP水平明显增高(P<0.05);与模型组比较,给药30天后,给药组大鼠的右足趾肿胀较模型组减轻(P<0.05),给药组大鼠血液、滑膜中TNF-α、SP及滑膜中IL一1β水平都显著降低(P<0.05),而脊髓后角中SP水平未见明显下降(P>0.05).结论:雷公藤多苷具有抗炎镇痛免疫抑制作用,其作用机制除降低血清和滑膜中的TNF-α、IL一1β水平外,还可能与降低佐剂性关节炎大鼠外周的SP水平有关,但对中枢SP无影响.

Influence of Tripterygium Polyglucoside on Substance P in Rats with Adjuvant Arthritis

Objective:To investigate the effects of tripterygium polyglucoside on substance P(SP)in adjuvant arthritis(AA) rats.Methods: AA rats were randomly divided into the normal control group,the model group,and the treatment group.Tumor necrosis factor-alpha(TNF-α)and interleukin-1beta(IL-1β)were used as indicators of inflammatory activity.The treatment group was intragastrically administered with tripterygium polyglucoside for 30 days after modeling.The levels of TNF-α,IL-1β,SP in peripheral sera and the synovial membrane and the level of SP in the spinal dorsal horn were measured by radioimmunoassay before and after treatment.SP level was compared among groups.Results: In the model group,the levels of SP in the spinal dorsal horn and TNF-α,IL-1β,SP in the blood and synovial membrane increased compared with those in the normal control group(P<0.05).After treatment with tripterygium polyglucoside,the swelling of the right toe was attenuated(P<0.05),and the levels of SP,TNF-α in the blood and synovial membrane and IL-1βin the synovial membrane were all lower in the treatment group,as compared with those in the model group(P<0.05).However,the level of SP in the spinal dorsal horn was not significantly different in the 2 groups after treatment(P>0.05).Conclusion:Tripterygium polyglucoside in the treatment of AA rats had favorable anti-inflammatory and analgesic effects that may involve reducing the levels of the peripheral SP besides TNF-α and IL-1β in the blood and synovial membrane,but had no effect on central SP.