甘草酸单铵配伍复方丹参注射液对大鼠盆腔粘连及组织生长因子的影响

目的:观察甘草酸单铵盐(MAG, monoammonium glycyrrhetate)配伍复方丹参注射液对大鼠子宫角粘连的预防作用及其作用机理.方法:制备大鼠宫角粘连模型,观察粘连评分,测定粘连组织中血管内皮生长因子(VEGF)、成纤维细胞生长因子(bFGF)的阳性表达率.结果:除复方丹参组外,各用药组均明显降低粘连评分和严重粘连百分率.与MAG组比较,MAG 复方丹参组、Dex组严重粘连百分率更低.与粘连组比较,MAG组、复方丹参组、MAG 复方丹参组、Dex组的VEGF、bFGF阳性细胞百分率均显著降低.结论:MAG、MAG配伍复方丹参可显著减少大鼠盆腔粘连的形成,尤以MAG配伍复方丹参组显著,二者可能具有协同作用,对粘连形成中晚期血管及纤维组织增生具有抑制作用可能是其作用机理之一.

Effect of monoammonium glycyrrhetate on peritoneal adhesions and VEGF and bFGF in rat

To evaluate the effects of monoammonium glycyrrhetate(MAG) and Compound Injection of Salvia militiorrhizam (CSM) on the prevention of peritoneal adhesions and the mechanism in rats. Method: The rat models of uterine horn adhesion was made according the references. The adhesion score and the rates of positve VEGF and bFGF of adhosion tissue were determined. Resuls: 1. Compared with NS group, CSM+MAG, MAG and Dex reduce the adhesion score (P<0.01)significantly except CSM alone. Compared with MAG, the effects of CSM +MAG and Dex significant reduced the percent of sever adhesion in tats (P<0.05). 2. Compared with NS group, the positive rate of VEGF and bFGF were significant reduced in CSM+MAG?MAG and Dex group. Conclusion: MAG alone or combined with CSM inhibit peritoneal adhision significantly.Combined with CSM, MAG has better effect than MAG alone. To reducing the positive expression of VEGF and bFGF is the potential mechanism of decreasing peritoneal adhesion of MAG.